Evidence map›Paper›PMID 40630004›Full record

ArticleScience progress

Embryonic stem cell-derived small extracellular vesicles alleviate lead-induced cochlear spiral ganglion neurons injury by activating PI3 K/AKT signaling pathway.

Lu Wang, Yijiang Bai, Yuanyuan Sun, Li Li, Ye He, GuoWei Li, Di Liu, Zhijun Huang, Jian Song, Hong Wu and 2 more

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Article in Science progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lu WangDepartment of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Yijiang BaiDepartment of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Yuanyuan SunDepartment of Reproductive Medicine, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
Li LiHunan Key Laboratory of Molecular Precision Medicine, Department of Oncology, Xiangya Hospital, Central South University, Changsha, China.
Ye HeDepartment of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
GuoWei LiDepartment of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Di LiuDepartment of Reproductive Medicine, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
Zhijun HuangDepartment of Reproductive Medicine, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
Jian SongDepartment of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Hong WuDepartment of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Hui LiDepartment of Reproductive Medicine, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
Xuewen WuDepartment of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.ORCID 0000-0001-7271-7965

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

ObjectiveEmbryonic stem cell (ESC)-derived small extracellular vesicles (sEVs) exhibit considerable potential as an innovative therapeutic approach in the fields of regenerative medicine and disease management. This in vitro study aimed to evaluate the protective efficacy of ESC-sEVs against lead acetate (PbAc)-induced damage in cochlear spiral ganglion neurons (SGNs).MethodsCochlear SGNs of neonatal rats were primarily cultured and administrated with (i) serum-free medium (control); (ii) 25 μM PbAc; (iii) 0.05 μg/μl ESC-sEVs (ESC-sEVs); (iv) 25 μM PbAc + 0.05 μg/μl ESC-sEVs (PbAc + ESC-sEVs). After treatment, cell viability of SGNs was assessed by CCK-8 assay. The changes in levels of reactive oxygen species, lipid peroxides, and apoptosis were detected by immunofluorescence and flow cytometry. The protective mechanisms of ESC-sEVs against lead-induced SGNs damage were elucidated by RNA sequencing and western blot analysis.ResultsThe findings revealed that ESC-sEVs significantly increased the viability of SGNs subjected to PbAc exposure. Immunofluorescence and flow cytometry revealed that ESC-sEVs effectively attenuated oxidative stress, lipid peroxidation, and apoptotic processes in PbAc-exposed SGNs. Furthermore, RNA sequencing and western blot analysis demonstrated that ESC-sEVs activated the PI3 K/AKT signaling pathway, which plays a pivotal role in alleviating lead-induced neuronal injury.ConclusionIn conclusion, this study provides the first evidence supporting the therapeutic potential of ESC-sEVs in addressing lead-induced ototoxicity.

Indexed as

Embryonic Stem CellsExtracellular VesiclesLeadNeuronsOrganometallic CompoundsPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSpiral GanglionAnimalsApoptosisCells, CulturedCell SurvivalRatsRats, Sprague-DawleyReactive Oxygen SpeciesSignal TransductionLeadlead acetateOrganometallic CompoundsPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktReactive Oxygen Speciesembryonic stem cellLead acetatePI3 K/AKT signaling pathwaysmall extracellular vesiclesspiral ganglion neurons

Identifiers

PMID40630004
PMCPMC12254551

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.