Evidence map›Paper›PMID 40629095›Full record

ArticleNature chemistry2025

The biophysical requirements that govern the efficient endosomal escape of designed mini-proteins.

Jonathan Giudice, Daniel D Brauer, Madeline Zoltek, Angel L Vázquez-Maldonado, Neville Dadina, Mark Kelly, Alanna Schepartz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Chemical Biology 2025: Highlights From the Ch/Bi145 Course at Caltech.Chembiochem : a European journal of chemical biology · 2026
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Jonathan Giudice *Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.ORCID 0000-0002-0775-9146
Daniel D Brauer *Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.ORCID 0000-0001-6974-3476
Madeline ZoltekDepartment of Molecular and Cellular Biology, University of California, Berkeley, Berkeley, CA, USA.ORCID 0000-0001-7988-7764
Angel L Vázquez-MaldonadoDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, USA.
Neville DadinaDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, USA.
Mark KellySchool of Pharmacy, University of California, San Francisco, San Francisco, CA, USA. mark.kelly@ucsf.edu.ORCID 0000-0003-3209-1018
Alanna SchepartzDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, USA. schepartz@berkeley.edu.ORCID 0000-0003-2127-3932

Funding

Fluorescence tools that illuminate biology and inspire translationR35GM134963 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Alanna Schepartz · 2020 to 2026
$4.7M
National Science Foundation (NSF) CHE-2203903National Science Foundation (NSF) CHE-221324National Science Foundation (NSF) DGE 2146752NIGMS NIH HHS R35 GM134963U.S. Department of Health & Human Services | National Institutes of Health (NIH) 1S10OD023455-01A1U.S. Department of Health & Human Services | National Institutes of Health (NIH) GM134963
6 · The paper itself

Abstract

The ZF5.3 mini-protein escapes endosomes efficiently to guide proteins into the cytosol and/or nucleus. However, other than the requirement for small or unfoldable cargo and an intact HOPS complex, little is known about how ZF5.3 traverses the limiting endocytic membrane. Here we characterize the requirements for efficient endosomal escape. We confirm that ZF5.3 remains folded at high temperatures and at pH values between 5.5 and 7.5. At lower pH, ZF5.3 unfolds cooperatively upon protonation of Zn(II)-binding His side chains whose pK

Indexed as

EndosomesHumansHydrogen-Ion ConcentrationLysophospholipidsMonoglyceridesbis(monoacylglyceryl)phosphateLysophospholipidsMonoglycerides

Identifiers

PMID40629095
PMCPMC13215133

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.