Evidence map›Paper›PMID 40628859›Full record

ArticleScientific reports2025

A single acute alcohol intoxication before fracture insult causes long-term elevated systemic RANKL and OPG levels in young adult mice.

Katrin Bundkirchen, Weikang Ye, Tianqi Zhang, Friederike Weidemann, Stefan Lienenklaus, Bastian Welke, Helen Rinderknecht, Borna Relja, Claudia Neunaber

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Katrin BundkirchenHannover Medical School, Department of Trauma Surgery, Hannover, Germany. Bundkirchen.Katrin@mh-hannover.de.
Weikang YeHannover Medical School, Department of Trauma Surgery, Hannover, Germany.
Tianqi ZhangHannover Medical School, Department of Trauma Surgery, Hannover, Germany.
Friederike WeidemannHannover Medical School, Department of Trauma Surgery, Hannover, Germany.
Stefan LienenklausHannover Medical School, Institute of Laboratory Animal Science, Hannover, Germany.
Bastian WelkeHannover Medical School, Department of Orthopaedic Surgery, Laboratory for Biomechanics and Biomaterials, Hannover, Germany.
Helen RinderknechtUlm University Medical Center, Department of Trauma, Hand, Plastic and Reconstructive Surgery, Translational and Experimental Trauma Research, Ulm, Germany.
Borna ReljaUlm University Medical Center, Department of Trauma, Hand, Plastic and Reconstructive Surgery, Translational and Experimental Trauma Research, Ulm, Germany.
Claudia NeunaberHannover Medical School, Department of Trauma Surgery, Hannover, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Binge drinking is the most common form of alcohol abuse and 25-40% of orthopedic trauma patients are intoxicated upon admission. While alcohol consumption prolongs the healing time of bone fractures, the underlying mechanisms by which alcohol leads to this fracture healing disorder remain unclear. 240 young adult and aged male C57BL/6J mice were examined. Half of the animals were acutely alcoholized two hours before surgery. The isolated fracture (Fx) group underwent an externally stabilized osteotomy and another group (THFx) an additional trauma hemorrhage (TH). The Sham group only received catheterization and fixation. Histological, radiological, biomechanical and systemic examinations were performed. Data were analyzed using two-way ANOVA and p ≤ 0.05 was considered significant. After 3 weeks, acute alcoholization led to increased receptor activator of NF-κB ligand (RANKL), osteoprotegerin (OPG) and RANKL/OPG ratio after fracture in young adult mice. In aged animals, this effect of alcohol was not present as values were already increased in the sober ones. More osteoblasts and macrophages were found in alcoholized young Fx animals. In conclusion, one-time binge drinking before trauma causes a long-term change in the RANKL/OPG ratio towards more RANKL in young adult mice.

Indexed as

Alcoholic IntoxicationFractures, BoneOsteoprotegerinRANK LigandAnimalsBinge DrinkingFracture HealingMaleMiceMice, Inbred C57BLOsteoprotegerinRANK LigandTnfrsf11b protein, mouseTnfsf11 protein, mouseBiomechanicsBone regenerationEthanolHemorrhagic shockHistologyµCT

Identifiers

PMID40628859
PMCPMC12238551

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.