Evidence map›Paper›PMID 40627773›Full record

ArticlePloS one2025

ECDD-S16, a synthetic derivative of cleistanthin A, suppresses pyroptosis in Burkholderia pseudomallei-infected U937 macrophages.

Suphasuta Khongpraphan, Sucharat Sanongkiet, Chularat Luangjindarat, Sarut Thairat, Bumrung Munyoo, Napason Chabang, Sitthivut Charoensutthivarakul, Suparerk Borwornpinyo, Patoomratana Tuchinda, Marisa Ponpuak and 2 more

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In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Suphasuta KhongpraphanDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Sucharat SanongkietDepartment of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand.
Chularat LuangjindaratDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Sarut ThairatOral Tissues, Cells and Molecular Biology Analysis and Research Center, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Bumrung MunyooExcellence Center for Drug Discovery (ECDD), Faculty of Science, Mahidol University, Bangkok, Thailand.
Napason ChabangSchool of Bioinnovation and Bio-Based Product Intelligence, Faculty of Science, Mahidol University, Bangkok, Thailand.
Sitthivut CharoensutthivarakulExcellence Center for Drug Discovery (ECDD), Faculty of Science, Mahidol University, Bangkok, Thailand.
Suparerk BorwornpinyoExcellence Center for Drug Discovery (ECDD), Faculty of Science, Mahidol University, Bangkok, Thailand.
Patoomratana TuchindaExcellence Center for Drug Discovery (ECDD), Faculty of Science, Mahidol University, Bangkok, Thailand.
Marisa PonpuakDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Pongsak UtaisincharoenDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Matsayapan PudlaDepartment of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.ORCID https://orcid.org/0000-0001-8807-4124

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMelioidosis is an infectious disease caused by an intracellular Gram-negative bacterium, Burkholderia pseudomallei, which is a common cause of community-acquired sepsis in Southeast Asia and Northern Australia. The mortality rate in acute melioidosis patients, which is caused by sepsis, is very high (47.1%). Therefore, reducing inflammation may lead to the treatment of patients with acute melioidosis. Previously, ECDD-S16 was reported to be a potential compound for inhibiting inflammatory cell death (pyroptosis).

objectiveIn this study, we further investigated the involvement of ECDD-S16 in pyroptosis induced by B. pseudomallei in the U937 human macrophage cell line.

methodsTo investigate the biological activity of ECDD-S16, U937 macrophages were infected with B. pseudomallei before treatment with the compound. The expression of pyroptosis marker was determined by lactate dehydrogenase (LDH) assay, western blotting and ELISA assay. Additionally, the intracellular growth of B. pseudomallei was examined by CFU determination. Furthermore, colocalization of the bacteria with phagosome acidification was observed by immunofluorescent staining.

resultsThe results showed that ECDD-S16 decreased LDH release and levels of pyroptosis-related proteins in B. pseudomallei-infected cells by inhibiting phagolysosome acidification. Moreover, the attenuation of pyroptosis did not interfere with the intracellular survival of B. pseudomallei in U937 macrophages.

conclusionOur findings indicated that ECDD-S16, a novel compound, interferes with caspase-1/4/5 activation, which may lead to the prevention of sepsis in acute melioidosis patients.

Indexed as

Burkholderia pseudomalleiMacrophagesMelioidosisPyroptosisHumansU937 Cells

Identifiers

PMID40627773
PMCPMC12237264

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