Evidence map›Paper›PMID 40627541›Full record

ArticleMicrobiology (Reading, England)2025

Difficult-to-culture micro-organisms specifically isolated using the liquid-liquid co-culture method - towards the identification of bacterial species and metabolites supporting their growth.

Atsushi Hisatomi, Takanobu Yoshida, Tomohisa Hasunuma, Moriya Ohkuma, Mitsuo Sakamoto

Abstract read
In one paragraph

Article in Microbiology (Reading, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Atsushi HisatomiMicrobe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan.
Takanobu YoshidaEngineering Biology Research Center, Kobe University, Kobe 657-8501, Nada, Japan.
Tomohisa HasunumaEngineering Biology Research Center, Kobe University, Kobe 657-8501, Nada, Japan.
Moriya OhkumaMicrobe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan.
Mitsuo SakamotoMicrobe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, the liquid-liquid co-culture method was applied using faecal samples and specific bacterial species as growth-supporting bacteria. We aimed to isolate new, difficult-to-culture bacterial species using metabolites produced by supportive bacteria to promote the growth of small bacteria selected using filter treatment. This study aimed to identify the supporting bacteria and their metabolites that promote the growth of these isolates. Analysis of the 16S rRNA gene sequences of the isolates obtained by co-culture revealed that they were

Indexed as

BacteriaAnimalsCoculture TechniquesCulture MediaDNA, BacterialFecesPhylogenyRNA, Ribosomal, 16SCulture MediaDNA, BacterialRNA, Ribosomal, 16Sco-culturehuman gutisolation methodmetabolomic analysissymbiotic relationshipWaltera intestinalis

Identifiers

PMID40627541
PMCPMC12282286

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.