Evidence map›Paper›PMID 40627168›Full record

ArticleApplied microbiology and biotechnology2025

Bacillus spore showcasing key antigens of African swine fever potentializes a promising oral vaccine candidate.

Xiaohua Wang, Baochao Fan, Tao Zhu, Hanwen Shen, Lian Li, Sihan Wang, Xiulian Yin, Baoxia Tian, Jinfeng Zhang, Bin Li and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. A three-plasmid-containing CRISPR-Cas9 platform to engineerApplied and environmental microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xiaohua Wang *Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, 223003, China.
Baochao Fan *Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Tao ZhuJiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, 223003, China.
Hanwen ShenJiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, 223003, China.
Lian LiJiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, 223003, China.
Sihan WangJiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, 223003, China.
Xiulian YinJiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, 223003, China.
Baoxia TianJiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, 223003, China.
Jinfeng ZhangJiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, 223003, China.
Bin LiJiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, 223003, China. libinana@126.com.
Chuping LuoJiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian, 223003, China. luochuping@163.com.ORCID http://orcid.org/0000-0002-3192-9889

Funding

Jiangsu Agricultural Science and Technology Innovation Fund CX(21)3127Jiangsu Provincial Key Research and Development Program BE2021324National Natural Science Foundation of China 32372619
6 · The paper itself

Abstract

African swine fever (ASF) remains a widespread and fatal infectious disease affecting both domestic pigs and wild boars around the world, and there is still no vaccine available for full protection. In recent years, Bacillus spores have been developed into effective vaccine carriers. Here, Bacillus amyloliquefaciens spores from strain CPLK1314 were used as a vaccine delivery system to display three highly antigenic proteins, p72, p30, and p54 of ASF, and their immune responses in BALB/c mice were evaluated. Our results demonstrated that the antibiotic-free Bacillus gene editing system constructed in this study can work effectively, and p72 and the fusion antigen p3054 were successfully expressed in the spore coat of CPLK1314 by fusing with CotB and CotG, respectively. Further animal experiments showed that the recombinant spores ΔBa72 and ΔBa3054 exhibited good immunogenicity, which induced high levels of antigen-specific serum IgG, proliferation of splenic lymphocytes, secretion of immune-related factors, as well as a significant increase in the proportion of T lymphocytes CD3

Indexed as

African Swine FeverAfrican Swine Fever VirusAntigens, BacterialBacillus amyloliquefaciensSpores, BacterialViral VaccinesAdministration, OralAnimalsAntibodies, ViralFemaleGene EditingImmunoglobulin GMiceMice, Inbred BALB CSwineAntibodies, ViralAntigens, BacterialImmunoglobulin GViral VaccinesAfrican swine feverBacillus amyloliquefaciensOral vaccineSpore surface display technology

Identifiers

PMID40627168
PMCPMC12238207

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.