ArticleJournal of veterinary emergency and critical care (San Antonio, Tex. : 2001)
Effect of N-Acetylcysteine, Ascorbic Acid, and a Vitamin E Analog on Oxidative and Storage Lesions in Canine Packed Red Blood Cells.
Article in Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed, 1 synthesis or guideline pooled it.
- From antidote to multimodal adjunct: a systematic review of N-acetylcysteine in dogs and cats.Frontiers in veterinary science · 2026Pooled it
- Donor- and unit-specific factors influencing hemolysis in stored canine and feline packed red blood cells.Frontiers in veterinary science · 2026Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo describe storage and oxidative lesions in canine packed red blood cells (pRBCs) during routine storage with additives, including saline, N-acetylcysteine (NAC), ascorbic acid (AA), and vitamin E analog (VE).
designProspective, comparative study of canine pRBCs with or without antioxidant additives during routine 42-day storage.
settingUniversity teaching hospital. ANIMALS: Nine leukoreduced units of canine pRBCs were aseptically separated into three aliquots (Groups 1, 2, and 3) on the same day as collection (day 0). All aliquots were shipped overnight and received by the investigators on day 1.
interventionsAntioxidants (or control solution) were added on day 1, with three treatment groups that included saline (control, Group 1), NAC and AA (Group 2), and AA and a VE (Group 3). MEASUREMENTS AND MAIN
resultsBlood was collected from each aliquot on day 1, before the addition of antioxidants for baseline measurement of glutathione and intraerythrocytic reactive oxygen species (ROS). Additional samples were collected from each aliquot on days 7, 28, and 42. Type 3 fixed-effects tests were used to compare the impact of group and time on each measurement. All groups showed storage lesions and glutathione depletion by day 42 compared with baseline, regardless of the antioxidant additive. Intraerythrocytic ROS accumulation was lower in Group 3 (AA and a VE) compared with other groups at all time points after baseline (p < 0.0001).
conclusionsThe addition of AA and a VE to canine pRBCs reduced ROS accumulation but did not prevent glutathione depletion during routine storage. Further studies using antioxidants as additives in canine pRBCs are warranted.
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