ArticleFrontiers in veterinary science2025
Development of nucleic acid-based RAA test strip assay for the rapid detection of foot-and-mouth disease virus.
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Developing a recombinase-aided amplification method combined with a lateral flow dipstick assay for rapid triplex detection of bovine coronavirus, infectious bovine rhinotracheitis virus, and bovine viral diarrhea virus.Microbiology spectrum · 2026Article
- Detection of duck adenovirus 3 using RAA-CRISPR/Cas12a based lateral flow dipstick method.Frontiers in microbiology · 2026Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Foot-and-mouth disease virus (FMDV) causes blister-like lesions in animals, leading to significant economic losses in animal husbandry. Accurate and rapid detection of FMD is crucial for effective prevention and control. The recombinase-aided amplification (RAA) technique enables rapid amplification of target fragments under isothermal conditions. In this study, based on the conserved sequence of the FMDV 3D gene, optimal RAA primers and probes were designed and screened, and a simple, rapid, and visual FMDV nucleic acid RAA test strip was developed. The optimum reaction conditions of the assay were determined to be 32°C for 30 min. The specificity, sensitivity, and repeatability of the FMDV nucleic acid RAA test strip were evaluated. The results demonstrated that the FMDV nucleic acid RAA test strip specifically reacted with FMDV nucleic acid and exhibited no cross-reactivity with other viruses. The lowest detection limits for recombinant plasmids and virus titer were 10 copies/μL and 10
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