Evidence map›Paper›PMID 40624981›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Efficient Somatic Cell Nuclear Transfer by Overcoming Both Pre- and Post-Implantation Epigenetic Barriers.

Yamei Li, Shiyu Sun, Yuting Xu, Jixiang Zhang, Yi Du, Yaxin Cao, Zhaodi Liao, Yali Xie, Xinyan Bian, Jiantao Huang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yamei LiState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Shiyu SunUniversity of Chinese Academy of Sciences, Beijing, 100049, China.
Yuting XuInstitute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.
Jixiang ZhangState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Yi DuState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Yaxin CaoState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Zhaodi LiaoUniversity of Chinese Academy of Sciences, Beijing, 100049, China.
Yali XieState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Xinyan BianInstitute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.
Jiantao HuangInstitute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.
Meijiao WangState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Zhen LiuUniversity of Chinese Academy of Sciences, Beijing, 100049, China.
Qiang SunUniversity of Chinese Academy of Sciences, Beijing, 100049, China.
Falong LuState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.ORCID https://orcid.org/0000-0002-8321-4818

Funding

Basic Frontier Scientific Research Program of CAS ZDBS-LY-SM019CAS Project for Young Scientists in Basic Research YSBR-012National Key Research and Development Program of China 2020YFA0804000National Key Research and Development Program of China 2024YFA1107900National Natural Science Foundation of China 31825018National Natural Science Foundation of China 82021001Shanghai Municipal Science and Technology Major Project 2018SHZDZX05Strategic Priority Research Program of Chinese Academy of Sciences XDB32060000
6 · The paper itself

Abstract

Mammalian cloning through somatic cell nuclear transfer (SCNT) involves reprogramming terminally differentiated cells into totipotent embryos. Epigenetic barriers inherited from somatic cells impede reprogramming efficiency and lead to low SCNT embryo development rates. Recent studies have identified two primary types of epigenetic barriers in mouse SCNT embryos, defective pre-implantation development due to abnormal gene expression around zygotic genome activation, associated with aberrant H3K9me3, H3K4me3, and histone acetylation, and defective post-implantation development due to loss of H3K27me3-mediated non-canonical imprinting. Despite these findings, effective strategies to overcome these barriers in a single embryo have not been established. Here, Kdm4d and Kdm5b overexpression are combined with TSA treatment to overcome epigenetic barriers in pre-implantation development, while using tetraploid complementation to replace extraembryonic lineage cells, thereby overcoming imprinting defects critical for post-implantation development. This approach resulted in ≈30% full-term development efficiency of reconstructed embryos. The strategy not only represents the highest SCNT efficiency achieved in mammals but also enhances the feasibility of efficient mammal cloning.

Indexed as

Cloning, OrganismEmbryonic DevelopmentEpigenesis, GeneticNuclear Transfer TechniquesAnimalsCellular ReprogrammingFemaleJumonji Domain-Containing Histone DemethylasesMiceJumonji Domain-Containing Histone Demethylasesembryoepigenetic barrierimprintinginner cell massSCNTtetraploid complementationtrophectoderm

Identifiers

PMID40624981
PMCPMC12499444

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.