Evidence map›Paper›PMID 40624899›Full record

ArticleCerebral cortex (New York, N.Y. : 1991)2025

Mapping of neurovascular and neurometabolic couplings by multimodal optical imaging.

Peter Herman, Simon Sanggaard, Shaun D James, Adil Akif, Sandeep Kumar Mishra, Basavaraju G Sanganahalli, Justus V Verhagen, Hal Blumenfeld, Fahmeed Hyder

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cerebral cortex (New York, N.Y. : 1991), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Peter HermanDepartment of Radiology and Biomedical Imaging, Yale University, 300 Cedar Street, New Haven, CT 06520, United States.
Simon SanggaardDepartment of Biomedical Engineering, 55 Prospect Street, Yale University, New Haven, CT 06511, United States.
Shaun D JamesDepartment of Radiology and Biomedical Imaging, Yale University, 300 Cedar Street, New Haven, CT 06520, United States.
Adil AkifDepartment of Biomedical Engineering, 55 Prospect Street, Yale University, New Haven, CT 06511, United States.
Sandeep Kumar MishraDepartment of Radiology and Biomedical Imaging, Yale University, 300 Cedar Street, New Haven, CT 06520, United States.
Basavaraju G SanganahalliDepartment of Radiology and Biomedical Imaging, Yale University, 300 Cedar Street, New Haven, CT 06520, United States.
Justus V VerhagenDepartment of Radiology and Biomedical Imaging, Yale University, 300 Cedar Street, New Haven, CT 06520, United States.
Hal BlumenfeldDepartment of Neuroscience, Yale University, 333 Cedar Street, New Haven, CT 06520, United States.
Fahmeed HyderDepartment of Radiology and Biomedical Imaging, Yale University, 300 Cedar Street, New Haven, CT 06520, United States.

Funding

Energetics of Neuronal Populations by fMRIR01MH067528 · NIMH · YALE UNIVERSITY · PI HYDER, DEWAN SYED FAHMEED · 2002 to 2021
$5.9M
Imaging the effects of transcranial alternatingcurrent stimulation in an experimental model of Alzheimer's DiseaseR01AG084681 · NIA · SUNNYBROOK RESEARCH INSTITUTE · PI Maged Goubran, Dewan Syed Fahmeed Hyder · 2024 to 2026
$1.7M
MRS validation of computational metabolic modeling of human brain function to determine energetic disruptions underlying fMRI-derived functional connectivity in degenerative or psychiatric disordersR01NS100106 · NINDS · YALE UNIVERSITY · PI HYDER, DEWAN SYED FAHMEED, ROTHMAN, DOUGLAS LYLE · 2017 to 2021
$959k
Impaired cerebral metabolism and blood flow by neuronal and astrocytic dysfunctions in Alzheimer's diseaseR21AG085366 · NIA · YALE UNIVERSITY · PI HERMAN, PETER · 2024 to 2025
$454k
National Institute of Health R01-AG084681National Institute of Health R01-MH067528National Institute of Health R21- AG085366NIA NIH HHS R01 AG084681NIA NIH HHS R21 AG085366NIMH NIH HHS R01 MH067528NINDS NIH HHS R01 NS100106
6 · The paper itself

Abstract

Neurovascular coupling links calcium (Ca2+)-dependent neuronal activity to cerebral blood volume changes, whereas neurometabolic coupling describes alterations of neuronal activity and glucose uptake. While mesoscale optical imaging of neurovascular coupling is prevalent, neurometabolic coupling has been explored much less. We describe a multiplexed optical system with a closed cranial window setup for longitudinal studies in Thy1-jRGECO1a mice where neuronal activity is measured with Ca2+-dependent red fluorescence, glucose uptake with bolus injections of 2NBDG with green fluorescence, and cerebral blood volume (CBV) with near-infrared spectroscopy (NIRS). Genetically encoded calcium indicators (GECIs) provide strong fluorescent signals for assessing Ca2+-dependent neuronal activity. Thy1-jRGECO1a, a novel GECI with red fluorescence emission that penetrates deeper into tissue, allows for simultaneous imaging of metabolic activity using a green-fluorescent glucose analog, 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2NBDG), which is taken up like glucose and then phosphorylated. Dual-fluorescent (red, green) and NIRS recordings confirm strong neurovascular coupling during hindpaw stimuli (Ca2+-CBV; P = 0.0033, r2 = 0.91), whereas neurometabolic coupling (Ca2+-2NBDG; P < 0.001) was three times stronger during stimulation (r2 = 0.75; slope = 0.6) compared to rest (r2 = 0.49; slope = 0.23). In summary, multiplexed optical imaging can be used to reveal mechanisms of neurovascular and neurometabolic (un)couplings during ischemia, traumatic brain injury, aging, and Alzheimer's disease.

Indexed as

BrainMultimodal ImagingNeurovascular CouplingOptical ImagingAnimalsCalciumCerebral Blood VolumeGlucoseMaleMiceMice, Inbred C57BLMice, TransgenicNeuronsSpectroscopy, Near-InfraredCalciumGlucose2NBDGaerobic glycolysisCa2+ imagingglucose consumptionnear-infrared spectroscopyThy1-jRGECO1a

Identifiers

PMID40624899
PMCPMC13070531

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.