Evidence map›Paper›PMID 40624731›Full record

ArticleGut microbes2025

The

Pritha Chatterjee, Vinicius Canale, Stephanie J King, Ali Shawki, Hillmin Lei, Alina N Santos, Michael Haddad, Casey Gries, Dermot P B McGovern, James Borneman and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gut microbes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Pritha ChatterjeeDivision of Biomedical Sciences, University of California, Riverside, Riverside, CA, USA.
Vinicius CanaleDivision of Biomedical Sciences, University of California, Riverside, Riverside, CA, USA.
Stephanie J KingDivision of Biomedical Sciences, University of California, Riverside, Riverside, CA, USA.
Ali ShawkiDivision of Biomedical Sciences, University of California, Riverside, Riverside, CA, USA.
Hillmin LeiDivision of Biomedical Sciences, University of California, Riverside, Riverside, CA, USA.
Alina N SantosDivision of Biomedical Sciences, University of California, Riverside, Riverside, CA, USA.ORCID 0000-0002-9041-7250
Michael HaddadDepartment of Biology, University of California, Riverside, Riverside, CA, USA.
Casey GriesDivision of Biomedical Sciences, University of California, Riverside, Riverside, CA, USA.
Dermot P B McGovernDepartment of Gastroenterology, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
James BornemanDepartment of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.
Declan F McColeDivision of Biomedical Sciences, University of California, Riverside, Riverside, CA, USA.ORCID 0000-0002-6286-0802

Funding

A NOVEL ROLE FOR PTPN2 IN INTESTINAL EPITHELIAL BARRIER REGULATIONR01DK091281 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Declan McCole · 2012 to 2026
$5.5M
Tyrosine Phosphatase Regulation of Mucosal Macrophage-Epithelial Cell Cross-talkR01AI153314 · NIAID · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI MCCOLE, DECLAN · 2020 to 2024
$2.5M
Targeted Identification of IBD Risk Gene Variant Impact on Immune-Epithelial Crosstalk and Intestinal Barrier FunctionR01DK138456 · NIDDK · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Declan McCole · 2024 to 2026
$1.3M
Inflammatory Bowel Disease Susceptibility Gene Regulation of AnemiaR21AI152017 · NIAID · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI MCCOLE, DECLAN · 2021 to 2022
$428k
NIAID NIH HHS R01 AI153314NIAID NIH HHS R21 AI152017NIDDK NIH HHS R01 DK091281NIDDK NIH HHS R01 DK138456
6 · The paper itself

Abstract

Inflammatory bowel disease (IBD) patients often exhibit expansion of the gut pathobiont, adherent-invasive E. coli (AIEC). Loss of activity of the IBD susceptibility gene, protein tyrosine phosphatase type 2 (PTPN2), causes gut microbiota dysbiosis in IBD patients, while Ptpn2 knock-out (Ptpn2-KO) mice display AIEC expansion. CEACAM6, a host cell surface glycoprotein, is exploited by AIEC to attach to and enter intestinal epithelial cells (IECs). Here, we investigated how IEC-specific PTPN2 restricts AIEC invasion. Intestinal biopsies from IBD patients heterozygous (CT) or homozygous (CC) for the PTPN2 SNP (single nucleotide polymorphism) rs1893217 were stained for CEACAM6. HT-29 IECs were transfected with control shRNA (PTPN2-CTL), or a shRNA targeted toward PTPN2 (PTPN2-KD). The rs1893217 SNP was inserted (PTPN2-KI), or a complete knock-out of PTPN2 (PTPN2-KO) was generated, by CRISPR-Cas9 gene editing of Caco-2BBe IECs. Adherence and invasion assays were performed with either the human IBD AIEC isolate, LF82, or a novel fluorescent-tagged mouse adherent-invasive E. coli (mAIEC

Indexed as

Antigens, CDCell Adhesion MoleculesInflammatory Bowel DiseasesProtein Tyrosine Phosphatase, Non-Receptor Type 2AllelesAnimalsEpithelial CellsGenetic Predisposition to DiseaseGPI-Linked ProteinsHT29 CellsHumansIntestinal MucosaJanus KinasesMiceMice, KnockoutPolymorphism, Single NucleotideAntigens, CDCEACAM6 protein, humanCell Adhesion MoleculesGPI-Linked ProteinsJanus KinasesProtein Tyrosine Phosphatase, Non-Receptor Type 2PTPN2 protein, humanSTAT Transcription FactorsAIECgut barrierIBDJAK-STATLF82tofacitinib

Identifiers

PMID40624731
PMCPMC12239772

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.