Evidence map›Paper›PMID 40624210›Full record

ArticleProtoplasma2026

Exploring effectiveness of salicylic acid and methyl jasmonate elicitors in influencing growth performance, epigenetic regulation, gene transcription, and secondary metabolism in pepper (Capsicum annuum): an in vitro biological assessment.

Zeinab Hasanvand, Alireza Iranbakhsh, Sara Saadatmand, Zahra Oraghi Ardebili

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Article in Protoplasma, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Zeinab HasanvandInstitute of Converging sciences and Technologies, SR.C., Islamic Azad University, Tehran, Iran.
Alireza IranbakhshInstitute of Converging sciences and Technologies, SR.C., Islamic Azad University, Tehran, Iran. iranbakhsh@iau.ac.ir.
Sara SaadatmandInstitute of Converging sciences and Technologies, SR.C., Islamic Azad University, Tehran, Iran.
Zahra Oraghi ArdebiliDepartment of Biology, Ga.C., Islamic Azad University, Garmsar, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Epigenetic responses of in vitro-cultured plants to elicitation with salicylic acid (SA) and methyl jasmonate (MJ) have been little explored. This study addresses how pepper seedlings respond to incorporating SA (0 and 10 µM) and/or MJ (0 and 10 µM) into the culture medium at physiological and molecular levels. The individual application of MJ or SA enhanced root fresh weight by an average of 59.8%. However, the mixed treatment synergistically augmented the root biomass by 2.2-fold. SA was individually more effective than MJ in triggering changes in the DNA methylome profiles. The epigenetic cytosine methylation in the MJ + SA group differed from that of the individual treatments. The individual SA treatment led to severe DNA hypermethylation, which was modified with the MJ application. Moreover, MJ and/or SA-mediated upregulation of the histone deacetylase (HDA) gene also confirmed the epigenetic regulation at the histone modification level. The MJ elicitor showed greater effectiveness in influencing the transcription of the target genes than the SA one. The expression of the MAPK kinase and ethylene-responsive transcription factor (EREB) genes displayed a similar upward trend. Applying pepper seedlings with MJ and SA increased proline concentrations and catalase activities. The simultaneous application of MJ and SA supplements effectively stimulated secondary metabolism, indicated by increased soluble phenols and activity of the phenylalanine ammonia-lyase enzyme. This study presents novel comparative insights into the SA and MJ-mediated epigenetic modifications. These findings can be employed to design in vitro culture systems and implement large-scale projects.

Indexed as

AcetatesCapsicumCyclopentanesEpigenesis, GeneticGene Expression Regulation, PlantOxylipinsSalicylic AcidSecondary MetabolismTranscription, GeneticDNA MethylationPlant RootsSeedlingsAcetatesCyclopentanesmethyl jasmonateOxylipinsSalicylic AcidDNA methylationElicitorEpigeneticGene regulationIn vitro culture

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.