Evidence map›Paper›PMID 40624161›Full record

ArticleCommunications medicine2025

A whole blood assay for antibody dependent phagocytosis of Plasmodium falciparum infected erythrocytes.

Dilini Rathnayake, Wina Hasang, Alexander Macpherson, HongHua Ding, Laurens Manning, Moses Laman, Maria Ome-Kaius, Holger W Unger, Feiko Ter Kuile, Mwayi Madanitsa and 4 more

Abstract read
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Article in Communications medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Dilini RathnayakeDepartment of Medicine (RMH), Peter Doherty Institute of Infection and Immunity, University of Melbourne, Melbourne, Vic, Australia.
Wina HasangDepartment of Infectious Diseases, Peter Doherty Institute of Infection and Immunity, University of Melbourne, Melbourne, Vic, Australia.
Alexander MacphersonDiscovery Chemistry Research and Technologies, Eli Lilly & Co., Bracknell, UK.
HongHua DingDepartment of Infectious Diseases, Peter Doherty Institute of Infection and Immunity, University of Melbourne, Melbourne, Vic, Australia.ORCID http://orcid.org/0000-0002-9738-5423
Laurens ManningUWA Medical School, The University of Western Australia, Perth, WA, Australia.
Moses LamanPapua New Guinea. Institute of Medical Research, Madang, Papua New Guinea.
Maria Ome-KaiusPapua New Guinea. Institute of Medical Research, Madang, Papua New Guinea.ORCID http://orcid.org/0000-0002-7094-474X
Holger W UngerGlobal and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Casuarina, NSW, Australia.
Feiko Ter KuileDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, UK.ORCID http://orcid.org/0000-0003-3663-5617
Mwayi MadanitsaDepartment of Clinical Sciences, Academy of Medical Sciences, Malawi University of Science and Technology, Thyolo, Malawi.
Bruce WinesImmune Therapies Group, Centre for Biomedical Research, Burnet Institute, Melbourne, Vic, Australia.ORCID http://orcid.org/0000-0002-4108-375X
P Mark HogarthImmune Therapies Group, Centre for Biomedical Research, Burnet Institute, Melbourne, Vic, Australia.
Elizabeth H AitkenDepartment of Infectious Diseases, Peter Doherty Institute of Infection and Immunity, University of Melbourne, Melbourne, Vic, Australia.ORCID http://orcid.org/0000-0002-2677-6208
Stephen J RogersonDepartment of Medicine (RMH), Peter Doherty Institute of Infection and Immunity, University of Melbourne, Melbourne, Vic, Australia. sroger@unimelb.edu.au.ORCID http://orcid.org/0000-0003-4287-1982

Funding

Department of Health | National Health and Medical Research Council (NHMRC) GNT1143946
6 · The paper itself

Abstract

backgroundAntibodies are used to protect against Plasmodium falciparum malaria. One antibody target, the variant surface antigens, is expressed on infected erythrocytes (IEs). Antibodies to these antigens can either block IE sequestration in the tissues, facilitate natural killer cell-mediated killing, or opsonise IEs for phagocytic clearance by neutrophils and monocytes.

methodsWe developed a high-throughput assay to measure antibody-dependent neutrophil phagocytosis (ADNP) and antibody-dependent cellular phagocytosis (ADCP, by blood monocytes) in the same sample of fresh whole blood.

resultsHere we show that immune plasma mediates ADNP and ADCP in a concentration-dependent manner. Uptake is greater in the presence of complement proteins and is largely dependent on the expression of P. falciparum Erythrocyte Membrane Protein 1 located on the IE surface. Plasma from pregnant Papua New Guinean women with and without placental malaria shows that ADNP and ADCP are associated with protection from placental malaria. ADNP, but not ADCP, using IEs expressing IT4VAR19 (a PfEMP1 variant that binds to endothelial protein C receptor through a DC8 domain cassette) is higher at hospital presentation in children with uncomplicated malaria than in severe malaria. In pregnant women, ADNP and ADCP in whole blood are strongly correlated with one another (Spearman's rho = 0.90), but not with ADNP or ADCP using purified neutrophils and monocytes in the absence of complement proteins.

conclusionsThe whole blood assay is a powerful new tool to assess functional antibodies that may protect against P. falciparum malaria. It allows simultaneous measurement of phagocytosis of opsonised IEs by monocytes and neutrophils.

Identifiers

PMID40624161
PMCPMC12234751

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.