Evidence map›Paper›PMID 40623986›Full record

ArticleBone research2025

Single-cell transcriptomic analysis identifies systemic immunosuppressive myeloid cells and local monocytes/macrophages as key regulators in polytrauma-induced immune dysregulation.

Drishti Maniar, M Cole Keenum, Casey E Vantucci, Tyler Guyer, Paramita Chatterjee, Kelly Leguineche, Kaitlyn Cheung, Robert E Guldberg, Krishnendu Roy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bone research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
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  3. Article
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  5. Review
  6. Pan-cancer analysis reveals TREM1Communications biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Drishti ManiarWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
M Cole KeenumWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Casey E Vantucci *Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Tyler Guyer *Department of Bioengineering, University of Oregon, Eugene, OR, USA.
Paramita ChatterjeeParker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.ORCID http://orcid.org/0000-0001-9761-2289
Kelly LeguinecheDepartment of Bioengineering, University of Oregon, Eugene, OR, USA.
Kaitlyn CheungWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Robert E GuldbergDepartment of Bioengineering, University of Oregon, Eugene, OR, USA.
Krishnendu RoyWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA. krish.roy@vanderbilt.edu.ORCID http://orcid.org/0000-0002-9972-0950

Funding

Immunoengineering Strategies for Musculoskeletal TraumaR01AR074960 · NIAMS · UNIVERSITY OF OREGON · PI GULDBERG, ROBERT E · 2020 to 2024
$2.5M
NIAMS NIH HHS R01 AR074960U.S. Department of Health & Human Services | National Institutes of Health (NIH) GR00008150
6 · The paper itself

Abstract

Polytrauma with significant bone and volumetric muscle loss presents substantial clinical challenges. Although immune responses significantly influence fracture healing post-polytrauma, the cellular and molecular underpinnings of polytrauma-induced immune dysregulation require further investigation. While previous studies examined either injury site tissue or systemic tissue (peripheral blood), our study uniquely investigated both systemic and local immune cells at the same time to better understand polytrauma-induced immune dysregulation and associated impaired bone healing. Using single-cell RNA sequencing (scRNA-seq) in a rat polytrauma model, we analyzed blood, bone marrow, and the local defect soft tissue to identify potential cellular and molecular targets involved in immune dysregulation. We identified a trauma-associated immunosuppressive myeloid (TIM) cell population that drives systemic immune dysregulation, immunosuppression, and potentially impaired bone healing. We found CD1d as a global marker for TIM cells in polytrauma. In the local defect tissue, we observed Spp1

Indexed as

Gene Expression ProfilingMacrophagesMonocytesMultiple TraumaMyeloid CellsSingle-Cell AnalysisTranscriptomeAnimalsMaleRatsRats, Sprague-Dawley

Identifiers

PMID40623986
PMCPMC12234669

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.