Evidence map›Paper›PMID 40623179›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Osteoinductive low-dose 3D porous calcium phosphate graphene oxide-integrated matrices enhance osteogenesis and mechanical properties.

Fatemeh S Hosseini, Taraje Whitfield, Jason D Orlando, Chenyun Deng, Amir A Abedini, Chrysoula Argyrou, Ho-Man Kan, Debolina Ghosh, Peter F Maye, Kevin W-H Lo and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Glucose metabolism in osteoporosis: A potential therapeutic target (Review).International journal of molecular medicine · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Fatemeh S HosseiniThe Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Farmington, CT 06030.ORCID 0000-0002-6941-6480
Taraje WhitfieldThe Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Farmington, CT 06030.
Jason D OrlandoDepartment of Chemistry, Carnegie Mellon University, Pittsburgh, PA 15213.
Chenyun DengDepartment of Chemistry, Carnegie Mellon University, Pittsburgh, PA 15213.
Amir A AbediniThe Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Farmington, CT 06030.
Chrysoula ArgyrouThe Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Farmington, CT 06030.
Ho-Man KanThe Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Farmington, CT 06030.
Debolina GhoshThe Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Farmington, CT 06030.ORCID 0000-0002-8681-9812
Peter F MayeDepartment of Skeletal Biology and Regeneration, UConn Health, Farmington, CT 06030.
Kevin W-H LoThe Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Farmington, CT 06030.
LakshmiS NairThe Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Farmington, CT 06030.
Stefanie A SydlikDepartment of Chemistry, Carnegie Mellon University, Pittsburgh, PA 15213.
Cato T LaurencinThe Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Farmington, CT 06030.ORCID 0000-0001-6765-3047

Funding

Regenerative Engineering of Musculoskeletal Tissues- a Convergence Doctoral Training ProgramT32AR079114 · NIAMS · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI LAURENCIN, CATO T. · 2021 to 2025
$1.1M
Skeletal, Craniofacial and Oral Biology Training GrantR90DE022526 · NIDCR · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI MINA, MINA · 2011 to 2022
$627k
HHS | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) T32 AR079114/NIAMS/NIHHHS | NIH | National Institute of Dental and Craniofacial Research (NIDR) 5R90DE022526NIAMS NIH HHS T32 AR079114NIDCR NIH HHS R90 DE022526
6 · The paper itself

Abstract

Bone regeneration continues to be a challenge due to the complex nature of the tissue. Identifying new materials that stimulate regeneration while providing mechanical properties is an active area of research. One class of promising material in bone regeneration is graphene and its derivatives including graphene oxide (GO), the oxidized form of graphene. Recently, calcium phosphate graphene (CaPG), synthesized from GO, has been proven to have osteoinductive properties in vivo. However, CaPG is a powder, and therefore, processing is difficult. Here, we present a method for creating porous CaPG matrices by incorporating it in poly(lactic-co-glycolic acid) (PLGA). This research has comprehensively evaluated CaPG-encapsulated PLGA-based microspheres for localized delivery of osteoinductive inducerons, calcium, and phosphate ions for bone regenerative engineering. CaPG distribution improved mechanical properties and matrix hydrophilicity. CaPG-integrated matrices successfully supported cell viability, proliferation, and enhanced osteogenic differentiation of MC3T3-E1 cells assessed by alkaline phosphatase activity and calcium deposition. Major osteogenic gene expression significantly increased, including Sp7, bone gamma-carboxyglutamate protein, COL1A1, bone sialoprotein, and dentin matrix protein1. The CaPG-containing matrices induced the endogenous canonical Wnt/β-catenin signaling pathway, with no significant difference when treated with DKK1 inhibition, demonstrating its possible selective activation mechanism. The increased protein expression of pathway-specific target genes, Bone morphogenic protein-2 (BMP-2) and WNT-1-inducible-signaling pathway protein 1 (WISP-1), further confirmed endogenous activation of canonical Wnt/β-catenin signaling. These results suggest that CaPG-modified matrices improve the cellular osteogenic differentiation via the canonical Wnt/β-catenin signaling pathway. This study provides further mechanistic insights into the nature of crosstalk between cells and inducerons-rich functionalized matrices for osteoblastic differentiation and improved bone regeneration.

Indexed as

BioengineeringBone RegenerationCalcium PhosphatesGraphiteAnimalsCell AdhesionCell LineCell ProliferationCell SurvivalMiceMicrospheresOsteogenesisWnt Signaling Pathwaycalcium phosphateCalcium Phosphatesgraphene oxideGraphitebone regenerationcalcium phosphategraphene oxidescaffoldWnt/β-catenin

Identifiers

PMID40623179
PMCPMC12280880

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.