Evidence map›Paper›PMID 40622627›Full record

ArticleActa parasitologica2025

Characterization of Calcifying Nanoparticles Isolated and Cultured in the Outer Capsule Wall of Hepatic Hydatid Cyst and their Correlation with Autophagy.

Haojie Shi, Sanqiang Niu, Xuanli Su, Furong Xu, Xiangwei Wu, Hongwei Zhang, Yuchang Wang, Jian Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta parasitologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Haojie Shi *The First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, 832000, China.
Sanqiang Niu *The First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, 832000, China.
Xuanli SuThe First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, 832000, China.
Furong XuThe First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, 832000, China.
Xiangwei WuThe First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, 832000, China.
Hongwei ZhangThe First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, 832000, China.
Yuchang WangTrauma Center, Department of Emergency and Traumatic Surgery, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P. R. China.
Jian YangThe First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, 832000, China. 125210525@qq.com.

Funding

Health Commission of Xinjiang Production and Construction Corps CZ001219The First Affiliated Hospital of Shihezi University BS202207The Science and Technology Bureau of the Corps 2022ZD090
6 · The paper itself

Abstract

purposeTo isolate calcifying nanoparticles (CNPs) from the outer capsule wall of hepatic hydatid cysts and determine their in vitro characteristics and potential link to autophagy-mediated inflammation.

methodsCNPs were isolated from 47 hepatic hydatid cyst walls and cultured for 4 weeks. The morphology and ultrastructure were examined using electron microscopy, and the elemental composition was determined using Energy-dispersive X-ray Microanalysis. Zeta potentials and particle size distributions were examined using a Zetasizer. The expression of autophagy-related proteins (LC3B, Beclin-1, and SQSTM1/P62) was analyzed using immunohistochemistry and western blotting.

resultsCNPs derived from the outer capsule wall of hepatic hydatid cyst exhibited similar properties to those of previously reported CNPs. The expression levels of Beclin-1 and LC3B were significantly elevated in calcified compared to non-calcified cyst walls (P < 0.05), whereas that of SQSTM1/P62 was higher in non-calcified walls (P < 0.05). The increased Beclin-1 and LC3B expression in calcified walls and SQSTM1/P62 in non-calcified walls (P < 0.01) were confirmed using immunohistochemistry. The content of CNPs was significantly higher in the calcified cyst walls (P < 0.001).

conclusionsCNPs can be isolated and cultured from the outer capsule wall of hepatic hydatid cysts, They are associated with autophagic activity in the outer capsule wall tissue and calcification of the cyst wall.

Indexed as

AutophagyCalcifying NanoparticlesEchinococcosis, HepaticAnimalsBeclin-1FemaleHumansImmunohistochemistryMaleBeclin-1Calcifying NanoparticlesAutophagyCalcificationCalcifying nanoparticlesElectron microscopyHepatic cystic echinococcosis

Identifiers

PMID40622627
PMCPMC12234601

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