Evidence map›Paper›PMID 40622534›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Cryptococcus neoformans and Microglia In Vitro Interaction as a Tool to Study Central Nervous System Infection.

Patrícia Albuquerque, André Moraes Nicola, Hugo Costa Paes

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Patrícia AlbuquerqueFaculty of Health Sciences and Technologies, University of Brasília, Brasília, DF, Brazil.
André Moraes NicolaFaculty of Medicine, University of Brasília, Brasília, DF, Brazil. amnicola@unb.br.
Hugo Costa PaesFaculty of Medicine, University of Brasília, Brasília, DF, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter presents in vitro protocols for studying interactions between Cryptococcus neoformans and microglial cells. These phagocytes are a critical component of the central nervous system's immune response to C. neoformans, the most common fungal agent of meningoencephalitis worldwide. Given its severity and high incidence in immunocompromised patients, understanding the interaction of C. neoformans with central nervous system (CNS) cells could help advance treatment strategies. These protocols leverage microscopy and molecular biology techniques to elucidate the mechanisms of C. neoformans invasion and persistence within the CNS, offering a methodological framework for fungal neuropathogenesis research.

Indexed as

Central Nervous System InfectionsCryptococcosisCryptococcus neoformansMicrogliaAnimalsHost-Pathogen InteractionsHumansCentral nervous systemCryptococcus neoformansHost-pathogen interactionMicrogliaPhagocytosis

Identifiers

PMID40622534

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.