In Vitro and In Vivo Models Studying Haemophilus influenzae.

Inesa Navasardyan, Rita Miwalian, Nicolette Golnazarian, Ishani Deshpande, Vishwanath Venketaraman

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Inesa NavasardyanCollege of Osteppathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA, USA.

Rita MiwalianCollege of Osteppathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA, USA.

Nicolette GolnazarianCollege of Osteppathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA, USA.

Ishani DeshpandeCollege of Osteppathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA, USA.

Vishwanath VenketaramanCollege of Osteppathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA, USA. vvenketaraman@westernu.edu.

Funding

Glutathione depletion in the CNS and its effects on Mtb infectionR15HL143545 · NHLBI · WESTERN UNIVERSITY OF HEALTH SCIENCES · PI VENKETARAMAN, VISHWANATH · 2019 to 2024

$852k

NHLBI NIH HHS R15 HL143545

6 · The paper itself

Abstract

Investigating the infiltration of Haemophilus influenzae into the central nervous system (CNS) involves exploring cellular migration across the blood-brain barrier (BBB). This study delves into established methodologies, encompassing both in vitro and in vivo approaches, to elucidate how H. influenzae disrupts the BBB, ultimately causing CNS infection. Moreover, the experiment sheds light on the impact of tumor necrosis factor (TNF)-α on zyxin levels. In the presence of cytokine TNF-α, human brain microvascular endothelial cells (HBMEC) exhibited a significant reduction in zyxin expression, accompanied by an increase in [specific effect]. Correspondingly, the introduction of H. influenzae as a cell-bound organism (CBO) in mice resulted in dire consequences for zyxin-deficient mice, emphasizing the critical role of zyxin in this context.

Indexed as

Haemophilus InfectionsHaemophilus influenzaeAnimalsBlood-Brain BarrierBrainDisease Models, AnimalEndothelial CellsHumansMiceTumor Necrosis Factor-alphaZyxinTumor Necrosis Factor-alphaZyxinBlood-brain barrier (BBB)Central nervous system (CNS)Haemophilus influenzaeTNF-αZyxin

Identifiers

PMID40622527

DOI10.1002/wsbm.166

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.