ArticleClinical and translational gastroenterology2025
Single GNAS Droplet-Based Digital Polymerase Chain Reaction Analysis of Pancreatic Cyst Fluid: An Effective Up-Front Strategy for Mucinous Cyst Diagnosis by Endoscopic Ultrasound-Guided Fine-Needle Aspiration.
Article in Clinical and translational gastroenterology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Pancreatic cystic lesions: position paper of the SIRM-AISP multidisciplinary group.La Radiologia medica · 2026Article
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16 authors.
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Abstract
introductionAccurate diagnosis of mucinous pancreatic cystic neoplasms (mPCNs) remains a clinical challenge. This study investigated the utility of single GNAS droplet-based digital polymerase chain reaction (ddPCR) analysis as a novel approach to refine the diagnostic accuracy of mPCNs using endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA).
methodsPatients who underwent EUS-FNA and GNAS pancreatic cyst fluid (PCF) analyses for pancreatic cystic lesion (PCL) assessment were prospectively enrolled. Cysts were categorized as mPCNs, non-mPCNs, or inconclusive PCLs (iPCLs) by integrating increasing information levels: high-resolution imaging and non-DNA PCF features (level 1), GNAS PCF analysis (level 2), and surgical pathology (level 3).
resultsOne hundred forty patients were included, 25 of whom underwent pancreatic surgery. Level 1 identified 68 mPCNs (49%), 24 non-mPCNs (17%), and 48 iPCLs (34%). GNAS mutations were detected in 42 of 68 (62%) mPCNs, 1 of 24 (4%) non-mPCNs, and 16 of 48 (33%) iPCLs. Level 2 increased mPCN detection to 62% and reduced iPCLs by one-third. Mutated GNAS showed 66% sensitivity for diagnosing mPCNs in the whole cohort and 65% in resected cases, outperforming both imaging and non-DNA PCF mucinous criteria, with 100% specificity and limited concordance with carcinoembryonic antigen, cytology, and fluid viscosity, highlighting its complementary diagnostic value. Cost-effectiveness simulations for iPCLs demonstrated that GNAS -ddPCR significantly reduced diagnostic costs by 24% compared with next-generation sequencing testing. DISCUSSION: Single GNAS- ddPCR analysis in PCF supported mPCNs diagnosis in 62% of cases and uncovered 33% of iPCLs as mPCNs with 100% specificity. It adds complementary value to standard cyst fluid markers offering a simple and cost-effective tool for improving PCL diagnosis by EUS-FNA.
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