Evidence map›Paper›PMID 40621164›Full record

ArticleFrontiers in cellular and infection microbiology2025

Multiplex droplet digital PCR for the detection and quantitation of

Yaling Cao, Junwen Wang, Yitong Jiang, Ling Xu, Yuan Tian, Zihao Fan, Zhenzhen Pan, Yinkang Mo, Xianru Zhu, Xiangying Zhang and 2 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Research progress on detection technologies for Mycoplasma pneumoniae.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Review
  2. Article
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yaling Cao *Beijing Institute of Hepatology/Beijing Youan Hospital, Capital Medical University, Beijing, China.
Junwen Wang *Department of Clinical Laboratory, Chui Yang Liu Hospital, Beijing, China.
Yitong Jiang *Yanjing Medical College, Capital Medical University, Beijing, China.
Ling XuBeijing Institute of Hepatology/Beijing Youan Hospital, Capital Medical University, Beijing, China.
Yuan TianBeijing Institute of Hepatology/Beijing Youan Hospital, Capital Medical University, Beijing, China.
Zihao FanBeijing Institute of Hepatology/Beijing Youan Hospital, Capital Medical University, Beijing, China.
Zhenzhen PanBeijing Institute of Hepatology/Beijing Youan Hospital, Capital Medical University, Beijing, China.
Yinkang MoBeijing Institute of Hepatology/Beijing Youan Hospital, Capital Medical University, Beijing, China.
Xianru ZhuBeijing Institute of Hepatology/Beijing Youan Hospital, Capital Medical University, Beijing, China.
Xiangying ZhangBeijing Institute of Hepatology/Beijing Youan Hospital, Capital Medical University, Beijing, China.
Jing HuangPeking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Department of Infection Control, Beijing, China.
Feng RenBeijing Institute of Hepatology/Beijing Youan Hospital, Capital Medical University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Lower respiratory tract infection is one of the major causes of disease and death worldwide. Methods: Specific primers and probes were designed for ddPCR. The sensitivity and specificity of the ddPCR assay were evaluated using standard strains, positive samples and 26 common pathogenic bacteria. One hundred and sixty-seven clinical samples were collected and tested via ddPCR, qPCR, bacterial culture and microfluidic chip technology. Results: The limits of detection (LoDs) of ddPCR were 2.5, 2.8 and 2.0 copies/μL for Conclusion: The multiplex ddPCR assay established in this study can accurately detect

Indexed as

Haemophilus InfectionsHaemophilus influenzaeMultiplex Polymerase Chain ReactionMycoplasma pneumoniaeStreptococcus pneumoniaeChild, PreschoolDNA, BacterialDNA PrimersHumansLimit of DetectionRespiratory Tract InfectionsSensitivity and SpecificityDNA, BacterialDNA PrimersddPCRHaemophilus influenzaemolecular diagnosisMycoplasma pneumoniaeStreptococcus pneumoniae

Identifiers

PMID40621164
PMCPMC12226287

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.