ArticleNature photonics2025
Electrochemical fluorescence modulation enables simultaneous multicolour imaging.
Article in Nature photonics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Illuminating the Interface: In Situ Optical Insights Into Two-Electron ORR Pathways for HAdvanced materials (Deerfield Beach, Fla.) · 2026Review
- Synthesis and Studies of BODIPY-Ferrocene Conjugates: An Overview.Chemistry, an Asian journal · 2026Review
- Integrating Electrochemistry and Optical Imaging to Probe Phospholipid Architectures: Emerging Paradigms in Fluorescence and Electrochemiluminescence Studies.Chemical & biomedical imaging · 2026Review
- Electrochemical Dye Switching Assisted Spectral Demixing and 3D STORM Imaging.Angewandte Chemie (International ed. in English) · 2026Article
- Electrochemically controlled switching of dyes for enhanced superresolution optical fluctuation imaging (EC-SOFI).Proceedings of the National Academy of Sciences of the United States of America · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Multicolour fluorescence imaging is crucial to simultaneously visualize multiple targets in cells, enabling the study of complicated cellular processes. Common multicolour methods rely on using fluorophores with sufficiently different spectral or lifetime characteristics. Here we present a new multicolour imaging strategy on a standard fluorescence microscope, where up to four fluorophores with high spectral overlap can be resolved using a single-colour optical configuration. We find that under electrochemical modulation, the fluorophores are regulated between the bright and dim states, with each displaying a distinct fluorescence response pattern. These unique fluorescence potential profiles enable the effective separation of different fluorophores through linear unmixing. We also demonstrate that electrochemical fluorescence switching is readily applicable to four-colour STED imaging. With no modification to the optical setups and easy adaptation to different microscopes, we anticipate that colour unmixing based on electrochemical fluorescence switching will provide an easily accessible multicolour imaging pathway for discoveries in diverse fields.
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Registered trials
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