Evidence map›Paper›PMID 40620804›Full record

ArticleBio-protocol2025

Activation of X-Succinate Synthases for Fumarate Hydroalkylation Using an In Vitro Activation Method.

Anshika Vats, Shukurah Anas, Ankush Chakraborty, Jian Liu, Jimyung Ryu, Sara M Mann, Mary C Andorfer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Anshika VatsDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Shukurah AnasDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Ankush ChakrabortyDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Jian LiuDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Jimyung RyuDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Sara M MannDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Mary C AndorferDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.

Funding

Investigation and application of hydrocarbon-degrading enzymes using cryo-electron microscopy and directed evolutionR00GM145910 · NIGMS · MICHIGAN STATE UNIVERSITY · PI ANDORFER, MARY CATHERINE · 2023 to 2025
$855k
Investigation and application of hydrocarbon-degrading enzymes using cryo-electron microscopy and directed evolutionK99GM145910 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI ANDORFER, MARY CATHERINE · 2022 to 2023
$112k
NIGMS NIH HHS K99 GM145910NIGMS NIH HHS R00 GM145910
6 · The paper itself

Abstract

X-succinate synthase enzymes (XSSs) are a class of glycyl radical enzymes (GREs) that play a pivotal role in microbial anaerobic hydrocarbon degradation. They catalyze the addition of hydrocarbons to fumarate using a protein-based glycyl radical, which must first be installed by a radical

Indexed as

4-isopropylbenzylsuccinate synthase (IBSS)Anaerobic hydrocarbon degradationBenzylsuccinate synthase (BSS)Fumarate additionGlycyl radical enzyme (GRE)Radical SAM activating enzyme

Identifiers

PMID40620804
PMCPMC12222630

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.