Evidence map›Paper›PMID 40618940›Full record

ArticleJournal of structural biology2025

Passage of ribosomes through microsprayer increases functional activity - Implications for activity assays in time-resolved cryo-EM.

Priyanka Garg, Xiangsong Feng, Swastik De, Joachim Frank

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of structural biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Priyanka GargDepartment of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, NY 10032, USA.
Xiangsong FengDepartment of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, NY 10032, USA.
Swastik DeDepartment of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, NY 10032, USA.
Joachim FrankDepartment of Biochemistry and Molecular Biophysics, Columbia University Irving Medical Center, New York, NY 10032, USA; Department of Biological Sciences, Columbia University, New York, NY 10027, USA. Electronic address: jf2192@cumc.columbia.edu.

Funding

Structural Studies of Macromolecular Assemblies Using Cryo-EMR35GM139453 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI FRANK, JOACHIM · 2021 to 2025
$3.9M
NIGMS NIH HHS R35 GM139453
6 · The paper itself

Abstract

This study examines the validity of an assay that is used to report on the retainment of functional competence by ribosomes as they pass a microsprayer. We find a reproducible increase, rather than the expected decrease in GFP production as monitored by fluorescence, which may suggest heterogeneity or partial aggregation of ribosomes in solution. An even larger increase in functional activity is observed when sonication is used, pointing to mechanical agitation as the decisive factor in both scenarios. The results have a bearing on the design and interpretation of validation experiments in time-resolved cryo-EM based on microfluidic chips.

Indexed as

Cryoelectron MicroscopyRibosomesGreen Fluorescent ProteinsSonicationGreen Fluorescent ProteinsCryo-EMFluorescenceMicrosprayerRibosomesSonicatorTime-resolved cryo-EM

Identifiers

PMID40618940
PMCPMC12399277

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.