Evidence map›Paper›PMID 40618137›Full record

ArticleBMC veterinary research2025

Isolation and characterization of Pseudomonas phage HJ01 and its therapeutic efficacy in canine pyoderma.

Yixian Wei, Ziyong Li, Jianlong Lao, Jijie Huang, Hailin Chen, Jiajia Li, Yayu Deng, Xinyu Mao, Runwen Ma, Yuxing Wu and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yixian Wei *College of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Ziyong Li *College of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Jianlong LaoCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Jijie HuangCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Hailin ChenCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Jiajia LiCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Yayu DengCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Xinyu MaoCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Runwen MaCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Yuxing WuCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Yizhou TanCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Xun LiCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Yangqing LuCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.
Shunjin JiangGuangdong Rongda Biological Co., Ltd, Qingyuan, Guangdong, 511500, China.
Xiaoye WangCollege of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China. xywang@gxu.edu.cn.

Funding

National Natural Science Foundation of China (NSFC) 32360902Natural Science Foundation of Guangxi Province 2021GXNSFAA196058The Key Research and Development of Wuming District 20210102
6 · The paper itself

Abstract

backgroundPseudomonas aeruginosa is a major pathogen in canine pyoderma, with increasing antibiotic resistance necessitating the development of alternative therapies. This study aimed to isolate and characterize a lytic phage targeting multidrug-resistant P. aeruginosa and evaluate its therapeutic efficacy in a canine pyoderma model. The key methods include isolation of phage HJ01 from wastewater, identification of genomic and biological characteristics, and subcutaneous injection of HJ01 in a canine pyoderma model induced by P. aeruginosa.

resultsThe host strain GDPA-01 exhibited multidrug resistance, with a 75% resistance gene detection rate, and harbored virulence genes such as exoU and exoT. Phage HJ01, classified as Pakpunavirus, exhibited optimal activity at pH 6.0-8.0, thermal stability up to 50 °C, a latent period of 20 min, and a burst size of 52 PFU/cell. In vivo, compared with the controls, HJ01 significantly reduced pruritus, cytology, and skin lesion scores (p < 0.05). Histopathology confirmed that in dogs treated with HJ01, epidermal repair was accelerated and inflammation was reduced.

conclusionsHJ01 emerges as a promising therapeutic candidate for managing multidrug-resistant P. aeruginosa infections in companion animals. This study pioneers the application of phage therapy in canine pyoderma, highlighting its potential to mitigate antibiotic overuse and address public health risks associated with zoonotic multidrug-resistant bacteria.

Indexed as

Dog DiseasesPhage TherapyPseudomonas aeruginosaPseudomonas InfectionsPseudomonas PhagesPyodermaAnimalsDogsDrug Resistance, Multiple, BacterialCanineMulti-drug resistant phage therapyPseudomonas aeruginosaPyoderma

Identifiers

PMID40618137
PMCPMC12228238

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.