ArticleJournal of nanobiotechnology2025
LUPP-LAMP: one-pot multiplatform universal LAMP assay for simultaneous detection and genotyping of DENV and ZIKV.
Article in Journal of nanobiotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Research Advances and Future Perspectives of Point-of-Care Detection Technologies and Biosensors for Mosquito-Borne Viruses.Biosensors · 2026Review
- Extraction-free LAMP-LFD assay for early diagnosis of congenital Chagas disease directly from neonatal dried blood spots.Frontiers in cellular and infection microbiology · 2026Article
- Isothermal real-time RT-RPA for Machupo virus detection: Field-adaptable sensitivity comparable with laboratory PCR.PloS one · 2026Article
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Authors and funding
5 authors.
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Abstract
Dengue virus (DENV) and Zika virus (ZIKV) are mosquito-borne viruses that cause severe health problems upon infection, necessitating timely and accurate diagnostic testing for effective prevention and control of their transmission. In this study, a novel multiplex nucleic acids detection method was introduced based on ligation-based universal primer and probe loop-mediated isothermal amplification (LUPP-LAMP) for the simultaneous detection and genotyping of DENV and ZIKV. By employing ligation of universal primers and probes using splint DNA ligase, LUPP-LAMP simplifies primer design, eliminates primer dimer formation, and achieves high sensitivity and specificity at low temperatures, with a detection limit of 10 copies/reaction. The robustness of this strategy allows for precise differentiation even at low concentrations and in cases of co-infection, which is crucial for addressing early diagnostic challenges due to similar symptoms. LUPP-LAMP is further adapted for point-of-care testing (POCT) by integrating lateral flow test strips and fluorescence imaging, offering rapid and user-friendly results in resource-limited settings. This method is also applied to DENV genotyping (serotypes 1-4) using melting curve analysis (MCA) in cases of co-infection, aiding in epidemiological surveillance and intervention strategies. In summary, LUPP-LAMP represents a significant advancement in the detection and genotyping of DENV and ZIKV, offering a versatile tool for laboratory and POCT applications, thereby enhancing public health outcomes in the control of mosquito-borne diseases.
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Registered trials
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