Evidence map›Paper›PMID 40618061›Full record

ArticleVirology journal2025

Metagenomics-based novel Caulimoviridae virus discovery and its development of identification markers in Lilium lancifolium thunb.

Huabing Liang, Yongxue Yang, Shuyuan Yi, Dingjun Cai, Haiping Zhou, Hongqing Yin, Chengxiao Yuan, Guolin Zhou, Daogang Tian, Xiaochuan Xia and 2 more

Abstract read
In one paragraph

Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Huabing LiangWuhan Academy of Agricultural Sciences, Wuhan, China.
Yongxue YangWuhan Academy of Agricultural Sciences, Wuhan, China.
Shuyuan YiWuhan Academy of Agricultural Sciences, Wuhan, China.
Dingjun CaiWuhan Academy of Agricultural Sciences, Wuhan, China.
Haiping ZhouEzhou Agricultural Science Research Institute, Ezhou, Hubei, China.
Hongqing YinEnshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi, China.
Chengxiao YuanEnshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi, China.
Guolin ZhouWuhan Academy of Agricultural Sciences, Wuhan, China.
Daogang TianEzhou Agricultural Science Research Institute, Ezhou, Hubei, China.
Xiaochuan XiaEzhou Agricultural Science Research Institute, Ezhou, Hubei, China.
Zhanchang WangWuhan Academy of Agricultural Sciences, Wuhan, China. 34801147@qq.com.
Fazhi ChenWuhan Academy of Agricultural Sciences, Wuhan, China. fazhch90@163.com.

Funding

Wuhan Academy of Agricultural Sciences innovation system science and technology strong foundation project- -Subject innovation project XKCX202405-3Wuhan metropolitan area science and technology strong chain project DOQ202402Wuhan science and technology correspondent "industry-university-research" special project 2023110201030689
6 · The paper itself

Abstract

Plant viruses cause considerable economic losses in the lily industry due to the emergence of diverse viral mutations and novel pathogens. Effective detection and identification of these viruses are critical for controlling their spread and mitigating infections. In this study, two novel Caulimoviridae viruses, namely Lancifolium Caulimovirus A (LCaA) and Lancifolium Caulimovirus B (LCaB), were identified for the first time in Lilium lancifolium Thunb. (tiger lily) through integrated metagenomic sequencing and Sanger sequencing. The complete genomes of LCaA and LCaB were determined to be 7,542 nt and 7,582 nt in length, respectively. Among the pooled tiger lily samples, seven LCaA isolates exhibited genome sequence identities ranging from 99.59 to 99.73%, while six LCaB isolates showed identities between 98.51% and 98.91%. Leveraging these isolates genomic variations, four sets of diagnostic markers were developed to distinguish the LCa viruses (LCaA and LCaB). Specific marker combinations were employed to identify LCa, LCaA, and LCaB strains. This study reports the discovery of two novel Caulimoviridae species and establishes a robust PCR-based methodology for their detection. The developed markers provide a valuable diagnostic tool for the early detection and management of LCa viruses in lily cultivation systems.

Indexed as

CaulimoviridaeLiliumMetagenomicsPlant DiseasesGenetic MarkersGenome, ViralPhylogenySequence Analysis, DNAGenetic MarkersCaulimovirusLilium lancifolium thunb.MetagenomicPCR markerVirus identification

Identifiers

PMID40618061
PMCPMC12228995

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.