Evidence map›Paper›PMID 40618048›Full record

ArticleBMC microbiology2025

Mouse models of aortic dissection induced by β-aminopropionitrile combined with angiotensin II and the changes in their gut microbiota.

Xinyu Hao, Shi Wu, Shuai Cheng, Xueling Wang, Jiawei Zhang, Xuan Li, Bo Jiang, Shijie Xin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xinyu Hao *Department of Vascular Surgery, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, Liaoning Province, China.
Shi Wu *Department of Vascular Surgery, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, Liaoning Province, China.
Shuai ChengDepartment of Endovascular Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan Province, China.
Xueling WangDepartment of Vascular Surgery, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, Liaoning Province, China.
Jiawei ZhangDepartment of Vascular Surgery, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, Liaoning Province, China.
Xuan LiDepartment of Vascular Surgery, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, Liaoning Province, China.
Bo JiangDepartment of Vascular Surgery, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, Liaoning Province, China.
Shijie XinDepartment of Vascular Surgery, The First Affiliated Hospital of China Medical University, China Medical University, Shenyang, Liaoning Province, China. sjxin@cmu.edu.cn.

Funding

Major Scientific and Technological Special Project of Liaoning Province 2022JH1/10400002
6 · The paper itself

Abstract

backgroundAortic dissection (AD) is a lethal vascular disease with no effective pharmacological treatments currently available. The construction of mouse models is crucial for researching the pathogenesis of AD. The gut microbiota play a role in the progression of AD, but studies on their changes in AD mouse models are lacking. This study compares various strategies for constructing mouse models of acute AD and examines changes in their gut microbiota.

resultsAcute AD models were created using male C57BL/6J mice aged 5-6 weeks, induced with β-aminopropionitrile (BAPN) combined with angiotensin II (Ang-II). The models differed in terms of dosage, timing, and path of administration. The treatment groups were compared in terms of general condition, blood glucose level, blood pressure, and rate of dissection formation and mortality. Hematological and histological changes of the tissues were analyzed. Changes in gut microbiota were determined through 16 S rDNA sequencing. All five strategies successfully induced AD in mice. BAPN at 1 g/kg/day combined with subcutaneous injection of Ang-II induced acute AD with higher incidence rates and lower mortality rate within 2 weeks. Regardless of the construction strategy, the AD mice showed similar changes in gut microbiota, displaying increased alpha and beta diversity. The relative abundance of Actinobacteria and Bacteroidetes increased and that of Firmicutes and Proteobacteria decreased. Ligilactobacillus, Escherichia, Enterobacteriaceae, and Gammaproteobacteria were dominant in the control group, whereas Muribaculaceae, Lactobacillus, Duncaniella, COE1, and Limosilactobacillus were dominant in the AD model group. Ligilactobacillus was significantly negatively correlated with the maximum diameters of the thoracic and abdominal aortas, whereas CAG-485 showed a significant positive correlation.

conclusionsBAPN at 1 g/kg/day combined with subcutaneous injection of Ang-II is a stable and efficient method to induce acute AD in mice. Dysbiosis occurred in all groups, with a high degree of consistency across construction strategies.

Indexed as

AminopropionitrileAngiotensin IIAortic DissectionGastrointestinal MicrobiomeAnimalsBacteriaDisease Models, AnimalMaleMiceMice, Inbred C57BLAminopropionitrileAngiotensin IIAngiotensin IIAortic dissectionExperimental mouse modelGut microbiotaβ-Aminopropionitrile

Identifiers

PMID40618048
PMCPMC12228179

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.