ArticleBMC microbiology2025
Evaluation of colistin susceptibility by four phenotypic methods compared to broth microdilution in multidrug-resistant Klebsiella pneumoniae.
Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- Review
- The colistin resistance pandemic: A dual threat from plasmid-encoded mobilised colistin resistance genes and chromosomal mutations.African journal of laboratory medicine · 2026Review
- Paeonol eradicates biofilm in porcine-source Escherichia coli by targeting the quorum sensing system.BMC veterinary research · 2025Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundColistin, one of the last-line treatment options for carbapenem-resistant microorganisms, presents challenges in determining its antimicrobial susceptibility. This study aims to compare colistin broth disk elution (CBDE), modified CBDE (mCBDE), the colistin agar test (CAT), and rapid polymyxin NP (RPNP) tests with the reference broth microdilution (BMD) in determining colistin susceptibility of 116 multidrug-resistant (MDR) Klebsiella pneumoniae isolates. Isolates were collected from blood and respiratory tract samples of different patients between 2022 and 2023 in Türkiye. For each isolate, a single 0.5 McFarland inoculum was prepared to inoculate CBDE, mCBDE, and CAT in parallel, then the suspension was adjusted to a density of 3-3.5 for RPNP. All tests were conducted simultaneously under the same incubation conditions. In the comparative analysis of the tests, bias, essential agreement (EA), and sensitivity were assessed according to ISO 20776-2:2021. Additionally, EA, categorical agreement (CA), major error (ME), and very major error (VME) were evaluated by the CLSI guidelines.
resultsThe minimum inhibitory concentration (MIC) values for MIC
conclusionThis study highlights the benefits of alternative phenotypic methods compared to reference BMD for assessing colistin susceptibility, including ease of use, cost-effectiveness, and rapid results. Our findings emphasize the need for more comprehensive multicenter studies to validate and improve these methods, especially those with more isolates with MIC values near the breakpoint.
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