ArticleScientific reports2025
Response surface optimization for cellulase production from Enterococcus faecium and Stutzerimonas stutzeri isolated from Gossypium arboretum and Solanum melongena soil.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rapid utilization of fossil fuel-based energy sources increased demand for alternate sustainable energy sources. One of the best alternate energy sources can be lignocellulosic biomass. The major constituent of lignocellulosic biomass is cellulose that can be converted into simple sugar using cellulase enzymes followed by fermentation for ethanol production. Two potential mesophilic cellulolytic bacteria, BS5 and CS7, from brinjal and cotton soil samples were screened based on high zones of hydrolysis on CMC agar plates and identified as Enterococcus faecium and Stutzerimonas stutzeri, respectively. It is the first instance of a mesophilic cellulase being reported from an S. stutzeri. CMCase production was enhanced by methods like one factor at a time (OFAT) and response surface methodology (RSM). The optimal conditions for maximum CMCase production by isolate BS5 were pH 5.0, 41℃, 1.25% inocula volume, and 56 h of incubation, whereas isolate CS7 produced maximum CMCase at pH 7.0, 43℃, 2.0% inocula volume, and 42 h of incubation. Following optimization through RSM-CCD, CMCase productivity of isolate BS5 increased 2.43 times, reaching 20.4 U/mL compared to 8.22 U/mL under unoptimized conditions, while CMCase productivity of isolate CS7 increased 2.18 times, reaching 24.08 U/mL compared to initial unoptimized activity of 11.05 U/mL. The crude enzyme produced by both isolates demonstrated effective potential in biopolishing cotton fabrics. Cotton fabrics treated with crude enzymes from BS5 and CS7 isolates lost 2.20% and 2.06% of their weight, respectively, showing that the enzymes removed tiny fibers from the surface of the cotton, making it smoother. Crude enzyme of both isolates showed optimum activity at mesophilic temperature, which makes them suitable for industrial applications like bioethanol production using simultaneous saccharification and fermentation, biopolishing and biostoning in the textile industry, and deinking in the paper and pulp industry.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.