Evidence map›Paper›PMID 40617881›Full record

ArticleScientific reports2025

Genome wide identification and characterization of EPFL genes and its potential association with male sterility in Brassica rapa.

Kang Wang, Yongkang Wang, Xiaoyan Tang, Na Liao, Danni Yang, Wenjie Wang, Lingyun Yuan, Jinfeng Hou, Jianqiang Wu, Chenggang Wang and 1 more

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Kang Wang *Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Yongkang Wang *Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Xiaoyan TangAnhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Na LiaoAnhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Danni YangAnhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Wenjie WangAnhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Lingyun YuanAnhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Jinfeng HouAnhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Jianqiang WuAnhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Chenggang WangAnhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Guohu Chen *Anhui Provincial Engineering Research Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China. cgh@ahau.edu.cn.ORCID https://orcid.org/0000-0002-6996-425X

Funding

Anhui Provincial Program on Major Basic Research Project 2023z04020005Excellent Young Talents Fund Program of Higher Education Institutions of Anhui Province 2024AH030017Natural Science Foundations of Anhui Province 2308085MC96Natural Science Foundations of Anhui Province 2308085QC101Natural Science Foundations of Hefei City 202343
6 · The paper itself

Abstract

The epidermal patterning factor-like (EPFL) gene family, encoding cysteine-rich polypeptides (CRPs), regulates diverse developmental processes in plants, including the control of stomatal density and the guidance of pollen tube elongation. To investigate its potential association with male sterility in Brassica rapa, we performed a comprehensive genome-wide identification using bioinformatics approaches. Our analysis revealed 14 BrEPFL members distributed across 8 of the 10 chromosomes. Phylogenetic analysis of EPFL proteins from 15 Brassicaceae species classified 204 orthologous genes into six distinct subfamilies, revealing their evolutionary conservation across crucifers. Syntenic collinearity and nonsynonymous/synonymous (Ka/Ks) analyses identified 63 orthologous gene pairs between B. rapa and three other species, all of which have undergone strong purifying selection during evolution. Tissue-specific expression profiling through RNA-seq and RT-qPCR demonstrated that most BrEPFL genes exhibit predominant expression in reproductive tissues. Co-expression network analysis implicated three BrEPFL coexpressed genes (BraA07g025640.3 C, BraA09g052700.3 C, and BraA10g002620.3 C) in pollen development pathways. These findings may provide valuable information for the further understanding of BrEPFL functions in male sterility in B. rapa.

Indexed as

Brassica rapaGenome, PlantPlant InfertilityPlant ProteinsChromosomes, PlantGene Expression ProfilingGene Expression Regulation, PlantPhylogenyPollenPlant ProteinsBrassica rapaComparative transcriptomic analysisEpidermal pattern building factor-likeMale sterility

Identifiers

PMID40617881
PMCPMC12228696

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