ArticleScientific reports2025
Impact of cryopreservation on DNA damage in Acer platanoides L. seeds evaluated by the comet assay.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Comparison of physical preservation strategies for accurate characterization of the coffee fruit microbiome.Archives of microbiology · 2026Article
- Cyclic voltammetry as a method for determining the viability of seeds: a case study on silver maple (Acer saccharinum L.).BMC plant biology · 2025Article
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Authors and funding
5 authors.
Funding
Abstract
Plant cells can suffer significant damage during cryopreservation, primarily due to extensive ice punctures and oxidative stress from reactive oxygen species (ROS). The extent of damage is closely linked to the moisture content (MC) of seeds, a crucial factor influencing their viability after cryostorage. However, there is limited information on the direct effects of MC in cryostored seeds on their overall (epi)genetic integrity. Maintaining genome integrity is essential for plant growth and ensures reliable transmission of genetic information. To investigate seed responses to genotoxic stress from desiccation and cryostorage, specific DNA damage, including DNA strand breaks, alkali-labile sites, and 8-oxo-7,8-dihydroguanine (8-oxoG), was measured, along with changes in epigenetic marks 5hmC (5-hydroxymethylcytosine) and 5mC (5-methylcytosine). This is the first study to demonstrate fine DNA strand breaks in orthodox seeds of Acer platanoides L. at varying MC immediately after cryostorage. Notably, 8-oxoG was detected for the first time in plant samples after cryostorage, and it was negatively correlated with seed viability, whereas the epigenetic marks showed a positive correlation. These findings indicate that cryostorage at non-optimal MC levels is genotoxic for seeds. Furthermore, the study demonstrated that the comet assay is a valuable technique for monitoring global DNA integrity in cryopreserved plant material.
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