Evidence map›Paper›PMID 40616836›Full record

ArticleChemistry & biodiversity2025

Antinociceptive and Anti-inflammatory Effect of Nicandrin B Isolated From Datura ferox in Zebrafish.

Jéssica Bezerra Maciel, Hortência Ribeiro Liberato, Antônio Wlisses da Silva, Maria Auxiliadora Solange Silva Gondim Pereira, Emanuela de Lima Rebouças, Francisco das Chagas L Pinto, Matheus Nunes da Rocha, Maria Kueirislene Amâncio Ferreira, Janaina Serra Azul Monteiro Evangelista, Izabel Florindo Guedes and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemistry & biodiversity, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Jéssica Bezerra MacielGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Hortência Ribeiro LiberatoGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.ORCID https://orcid.org/0000-0001-5527-164X
Antônio Wlisses da SilvaGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Maria Auxiliadora Solange Silva Gondim PereiraGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Emanuela de Lima RebouçasGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Francisco das Chagas L PintoDepartment of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Brazil.
Matheus Nunes da RochaGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Maria Kueirislene Amâncio FerreiraGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Janaina Serra Azul Monteiro EvangelistaLaboratory of Comparative Experimental Morphology - FAVET Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil.
Izabel Florindo GuedesLaboratory of Biotechnology and Molecular Biology, Health Sciences Center, State University of Ceará, Fortaleza, Brazil.
Marcia Machado MarinhoGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Emmanuel Silva MarinhoGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Andreia Ferreira de Castro GomesDepartment of Biology, Environmental Biology, School of Sciences, University of Minho, Braga, Portugal.
Otília Loiola PessoaDepartment of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Brazil.
Jane Eire Silva Alencar de MenezesGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Hélcio Silva Dos SantosGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nicandrin B (Nic B), a withanolide isolated from Datura ferox leaves, was investigated for its antinociceptive and anti-inflammatory effects in adult zebrafish (Danio rerio). Animals treated with Nic B (4, 20, and 40 mg/kg) showed no toxicity and maintained normal locomotor activity. The compound significantly reduced nociception induced by formalin and hypertonic saline; these effects were reversed by TRPA1 and transient receptor potential vanilloid 1 antagonists, indicating neuromodulation of these targets. Additionally, Nic B attenuated carrageenan-induced abdominal edema, reduced neutrophil recruitment, and decreased hepatic reactive oxygen species levels. Docking analysis confirmed a favorable binding affinity with TRPA1, supporting its therapeutic potential. These findings suggest that Nic B combines analgesic, anti-inflammatory activity, and toxicological safety, making it a promising drug candidate.

Indexed as

AnalgesicsAnti-Inflammatory AgentsAnti-Inflammatory Agents, Non-SteroidalAnimalsCarrageenanDose-Response Relationship, DrugEdemaMaleMolecular Docking SimulationMolecular StructureReactive Oxygen SpeciesStructure-Activity RelationshipTRPA1 Cation ChannelZebrafishAnalgesicsAnti-Inflammatory AgentsAnti-Inflammatory Agents, Non-SteroidalCarrageenanReactive Oxygen SpeciesTRPA1 Cation ChannelanalgesiainflammationreceptorsTRPA1 channelsTRPV1 channelvitanolide

Identifiers

PMID40616836
PMCPMC12629155

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.