Evidence map›Paper›PMID 40616727›Full record

ArticleApplied biochemistry and biotechnology2025

The Combination of UPLC MS and Derivatization Method for Analysis of Purslane for Ulcerative Colitis In Vivo.

Xudong Li, Mingxuan Cui, Lijie Zhu, Haoqian Zhang, Yunhui Guan, Xiangjia Song, MengLin Zhao, Zhiguo Li, Fei Liao, Liqiang Shi and 1 more

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Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Xudong LiCollege of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, China.
Mingxuan CuiCollege of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, China.
Lijie ZhuCollege of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, China.
Haoqian ZhangCollege of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, China.
Yunhui GuanCollege of Pharmacy, Beihua University, Jilin, 132013, China.
Xiangjia SongCollege of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, China.
MengLin ZhaoCollege of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, China.
Zhiguo LiCollege of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, China.
Fei LiaoCollege of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, China. liaofeiwower@163.com.
Liqiang ShiCollege of Pharmacy, Beihua University, Jilin, 132013, China. m18744032009@163.com.
Ningning ZhaoCollege of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, China. zhaonn92@163.com.

Funding

China Central Government Guides Local Science and Technology Development Foundation 236Z7712GNational Natural Science Foundation of China 82204604Natural Science Foundation of Hebei Province H2020209243Natural Science Foundation of Hebei Province H2022209012Natural Science Foundation of Jilin Province YDZJ202401686ZYTSthe Program of Hebei Provincial Education Department BJK2024039Youth Scholars Promotion Plan of North China University of Science and Technology QNTJ202309
6 · The paper itself

Abstract

Portulaca oleracea L. (PL) has significant clinical efficacy in treating ulcerative colitis (UC), which significantly affects patients' daily life. However, the absorption and metabolism of PL in vivo remain unclear due to the difficulty in detecting organic acids. This study focused on analyzing the pharmacogenetic composition of PL extract in 24 rats (n = 6) and its targeted network pharmacology. Using UPLC MS with derivatization techniques, plasma, and faeces were collected from healthy and UC rats gavaged with PL extracts. Subsequently, in vitro derivatization of the above biological samples and PL extracts was performed before and after derivatization to qualitatively analyze to ensure biosafety. A total of 61 active constituents and 21 constituents in vivo were detected. After derivatization, the sensitivity for detecting non-acid compositions increased in the negative mode, and the sensitivity for detecting acids also increased in the positive mode. For plasma and intestinal analysis, the detective sensitivity of organic acid was effectively increased for 1-3 times, and healthy rats with PL were detected with higher levels of the active constituents than that of UC rats with PL. Combined with the pharmacological analysis of the targeting network, it might act through the hypoxia-inducible factor-1 (HIF-1) signaling pathway and phosphatidylinositol 3-kinase (PI3K-Akt) signaling pathway. However, individual differences in the samples may limit the generalizability of the results, and further validation with larger sample sizes is needed. This study not only provides a scientific basis for the material basis of the medicinal effects but also provides new ideas and methods for the study of traditional Chinese medicines.

Indexed as

Colitis, UlcerativeMass SpectrometryPlant ExtractsPortulacaAnimalsChromatography, High Pressure LiquidMaleRatsRats, Sprague-DawleyPlant ExtractsDerivatizationIn vivo composition analysisPurslane oleracea L.Ulcerative colitisUPLC MS

Identifiers

PMID40616727

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