ArticleBMC nephrology2025
Application of low-vacuum scanning electron microscopy and energy-dispersive X-ray spectrometry for detection of renal tubular crystals: a case of nephrocalcinosis in the setting of anorexia nervosa.
Article in BMC nephrology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Low-vacuum scanning electron microscopy for informative three-dimensional imaging of cell/tissue architectures and biomedical target localization.Microscopy (Oxford, England) · 2026Review
- Decoding the Spectrum of Anorexia Nervosa: Clinical Impact, Molecular Insights, and Therapeutic Perspectives.Biomolecules · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
backgroundNephrocalcinosis, characterized by excessive calcium deposition within the renal parenchyma, can be a long-term complication of anorexia nervosa (AN). The extent of renal calcification can range from molecular-level alterations to macroscopic stone formation, contributing to progressive kidney injury. While early detection is critical for prognosis, limited studies have characterized the structural and elemental composition of renal calcifications associated with AN. Here, we report a case of nephrocalcinosis in the setting of AN. CASE PRESENTATION: A 25-year-old woman with AN presented with progressive renal dysfunction and gouty arthritis. Renal biopsy revealed focal tubulointerstitial nephritis with extensive calcification. Conventional light microscopy identified crystalline deposits within the tubular lumen, but further characterization required advanced imaging techniques. Low-vacuum scanning electron microscopy (LV-SEM) of formalin-fixed paraffin-embedded (FFPE) specimens demonstrated more widespread crystalline deposits than were visible by routine histopathology. Elemental analysis via energy-dispersive X-ray spectrometry (EDX) confirmed that the deposits were primarily composed of calcium phosphate.
conclusionsThese findings underscore the utility of LV-SEM/EDX in detecting and characterizing renal calcifications at high resolution. The ability to perform elemental analysis on FFPE tissue sections provides a novel and practical approach for elucidating the pathogenesis of nephrocalcinosis. These methods may enhance the accuracy of renal crystal identification, thereby facilitating earlier diagnosis and improved management of nephrocalcinosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.