ArticleArthritis research & therapy2025
IFNγ production during cell interactions distinguishes localized from diffuse pigmented villonodular synovitis and rheumatoid arthritis.
Article in Arthritis research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPigmented villonodular synovitis (PVNS) is a rare articular disease characterized by aggressive synovial proliferation, with localized or diffuse forms. PVNS shares features of an inflammatory disease such as rheumatoid arthritis (RA), including immune cell infiltrate. Thus, we aimed to evaluate PVNS synoviocyte response to inflammatory stimulation or cell interactions to better understand their role in pathophysiology. Results were compared with those in RA.
methodsSynoviocytes were treated with pro-inflammatory cytokines, IL-17 and/or TNF. IL-6 and IL-8 production was evaluated by ELISA in culture supernatants after 48 h. Migratory capacity was evaluated by a cell scraping assay. Peripheral blood mononuclear cells (PBMC) from healthy donors were co-cultured with PVNS or RA synoviocytes during 48 h, in the presence or not of phytohemagglutinin (PHA). Cytokine production (IL-17, IL-6, IFN-γ, IL-10, IL-1β and TNF) was measured by ELISA.
resultsThe addition of IL-17 and TNF stimulated IL-6 and IL-8 secretion by both PVNS and RA synoviocytes, with similar responses between PVNS and RA synoviocytes. The highest production of IL-6 and IL-8 was obtained with the combination of IL-17 + TNF. Diffuse PVNS synoviocytes were less potent to cover a scratch area than localized PVNS or RA synoviocytes (p < 0.05). Finally, responses to cell interactions were assessed using co-cultures between synoviocytes and activated immune cells. IL-17, IL-6, IFNγ, IL-10, IL-1β and TNF production was measured after 48 h. Cell interactions induced massive cytokine production, mainly in PHA activated condition. The source of stromal cells affected the secretion resulting from these interactions. Localized and diffuse PVNS synoviocytes induced more IL-17 than RA synoviocytes (p ≤ 0.01). Localized PVNS induced more IFNγ than both diffuse PVNS and RA synoviocytes (p ≤ 0.05). IL-10 production was negatively correlated with IFNγ secretion.
conclusionIn conclusion, results show differences in synoviocyte profiles or in response to cell interactions depending on synoviocyte source, with changes in IFNγ / IL-10 balance associated with localized PVNS. These differences could be used to adapt the therapeutic strategy to each form of PVNS.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.