Evidence map›Paper›PMID 40613993›Full record

ArticleRice (New York, N.Y.)2025

Overexpression of the ABA Synthesis Gene OsABA2 Enhances Seed Storability in Rice.

Yongxiang Liao, Pengfei Jiang, Mingming Zhang, Fangli Jin, Mengyuan Li, Jiazhi Dong, Daiming Guo, Yingxiu Liao, Asif Ali, Saira Saleem and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Rice (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Yongxiang Liao *State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Pengfei Jiang *State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Mingming Zhang *State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Fangli JinState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Mengyuan LiState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Jiazhi DongState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Daiming GuoState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Yingxiu LiaoState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Asif AliState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Saira SaleemOilseeds Research Station, Ayub Agricultural Research Institute, Khanpur, 38850, Faisalabad, Pakistan.
Xiangyang ZhangBranch of China National Hybrid Rice Research and Development Centre, Sichuan Tiland Huizhi Biology Science and Technology Co., Ltd., Chengdu, 611730, China.
Qinggui JiangSeed Management Station, Sichuan Provincial Department of Agriculture and Rural Affairs, Chengdu, 610041, China.
Su LiuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Hongyu ZhangState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Xiaoqiong ChenState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Kangxi DuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Hao ZhouState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Duo XiaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Peizhou XuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Xianjun WuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. wuxj@sicau.edu.cn.

Funding

Sichuan Provincial Science and Technology Support Program 2022ZDZX0016, 2021YFYZ0016, 2023NSFSC0013).
6 · The paper itself

Abstract

Seed storability is critical for seed production and germplasm conservation. Numerous studies have linked seed storability to abscisic acid (ABA) metabolism; however, the use of biotechnological approaches to strategically and rapidly enhance seed storability in rice through manipulation of ABA biosynthesis genes remains unexplored. In the current study, we developed overexpression lines (OE) of OsABA2, which encodes a XANTHOXIN DEHYDROGENASE, a key enzyme in the ABA synthesis pathway. Our experimental results showed that the relative expression of OsABA2 was upregulated in response to artificial aging treatment. The germination rate of OsABA2-OE lines was significantly higher, while the electrical conductivity of the seed leachate was lower compared to the wild type (WT), indicating improved seed vigor. Following artificial aging treatments, OsABA2-OE lines exhibited less pronounced changes in storage substances such as sugars and total starch, relative to WT. Reduced diaminobenzidine (DAB) staining intensity in OsABA2-OE lines suggested lower levels of reactive oxygen species (ROS). Correspondingly, the contents of hydrogen peroxide and malondialdehyde were lower, whereas catalase activity and total antioxidant capacity were higher in OsABA2-OE lines after artificial aging treatments. Comparative transcriptome analysis further revealed that the overexpression of OsABA2 may enhance seed storability by modulating the expression of ROS scavenging genes. For practical application, Gang46B-a hybrid rice parental line with poor storability-significantly improved its post-aging germination rates. These findings demonstrate that the overexpression of OsABA2 enhances seed storability by regulating ABA biosynthesis pathway and associated oxidative response. Thus, OsABA2 represents a promising molecular target for precise improvement of seed storage traits. This approach could be utilized for the improvement of seed storability in rice and other crops, offering valuable implications for the seed industry.

Indexed as

Abscisic acidArtificial aging treatmentOsABA2ROSSeed storability

Identifiers

PMID40613993
PMCPMC12227392

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.