ArticleParasite (Paris, France)2025
Usefulness of microsatellite loci for differentiating between Dibothriocephalus dendriticus and Dibothriocephalus ditremus (Cestoda: Diphyllobothriidea).
Article in Parasite (Paris, France), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Dominance ofCurrent research in parasitology & vector-borne diseases · 2026Article
- Occurrence and the first molecular genotyping ofCurrent research in parasitology & vector-borne diseases · 2025Article
- Dibothriocephalosis in salmonids from Iceland: A more complex taxonomic problem than assumed until now?Current research in parasitology & vector-borne diseases · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Differentiating between two diphyllobothriid tapeworms Dibothriocephalus dendriticus and Dibothriocephalus ditremus is complicated due to their morphological plasticity, intraspecific variability and a wide range of common hosts. The aim of this study was to develop a species-specific PCR-based method for single-step discrimination between D. dendriticus and D. ditremus. Intraspecific variation and interspecific differences were analysed in subunits/spacers of nuclear rRNA genes and protein-coding genes of mitochondrial DNA. In addition, the specificity of primers designed for the amplification of microsatellite loci in D. dendriticus was tested on D. ditremus DNA. Due to high identity within the rRNA gene in these species, no suitable DNA regions could be identified for the design of the species-specific primers. A higher level of interspecific differences was detected in the mitochondrial cox1 and cob genes, in which regions containing species-specific mutations were chosen for the design of D. dendriticus- and D. ditremus-specific primers. However, their specificity was not confirmed, as the D. dendriticus-specific primers also annealed to D. ditremus DNA and vice versa. Of the 15 primer pairs designed for the amplification of microsatellite loci in D. dendriticus, 13 primer pairs also annealed to D. ditremus DNA. Only two primer pairs, which amplify the Dd_8 and Dd_33 loci have been proven to be D. dendriticus-specific. The effectiveness and high reproducibility of the Dd_8 primers were validated on ~3,500 D. dendriticus and D. ditremus plerocercoids from Iceland and Norway. These primers are recommended for future molecular differentiation between both Dibothriocephalus species.
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