Evidence map›Paper›PMID 40612902›Full record

ArticleiScience2025

STT3A-mediated mega protein complex assembly during dengue and Zika virus infection.

Tao Liu, Natasha W Hanners, Huangheng Tao, Claudia Szabo, Dao Xu, Wei Lin, John W Schoggins, Nan Yan, Jianjun Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Tao LiuCenter for Immunotherapy & Precision Immuno-Oncology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Natasha W HannersDepartment of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Huangheng TaoCenter for Immunotherapy & Precision Immuno-Oncology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Claudia SzaboCenter for Immunotherapy & Precision Immuno-Oncology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Dao XuCenter for Immunotherapy & Precision Immuno-Oncology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Wei LinDepartment of Mathematics and Statistics, Cleveland State University, Cleveland, OH 44115, USA.
John W SchogginsDepartment of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Nan YanDepartment of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Jianjun WuCenter for Immunotherapy & Precision Immuno-Oncology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.

Funding

A functional evolutionary genetic approach to combat viral infectionDP1AI158124 · NIAID · UT SOUTHWESTERN MEDICAL CENTER · PI SCHOGGINS, JOHN W. · 2020 to 2024
$5.7M
Interferon-mediated control of neuropathogenic FlavivirusesK08AI132751 · NIAID · UT SOUTHWESTERN MEDICAL CENTER · PI HANNERS, NATASHA WYNDHAM · 2019 to 2023
$744k
NIAID NIH HHS DP1 AI158124NIAID NIH HHS K08 AI132751NIAID NIH HHS L40 AI140376
6 · The paper itself

Abstract

Flavivirus replication in mammalian cells requires host oligosaccharyltransferase (OST) complex, which is classically known to catalyze protein N-glycosylation. However, enzymatic activity of OST is not required for flavivirus infection, leaving the underlying mechanism puzzling. We show the STT3A sub-complex of OST, including STT3A and DC2, to be critically required for dengue virus (DENV) and Zika virus (ZIKV) infection. We find that STT3A nucleates a mega protein complex assembly during DENV infection as a scaffold through its interaction with other OST subunits, translocon proteins, and viral nonstructural proteins. The integrity of this mega protein complex is important for supporting flavivirus infection. We also identified a small-molecule compound NSC-323241 that disrupts STT3A-mediated mega protein complex assembly and potently blocks DENV and ZIKV infection. Together, our study reveals a scaffolding function of STT3A in flavivirus infection through comprehensive molecular dissection of the multi-subunit OST complex and associated host and viral proteins.

Indexed as

Biological sciencesMicrobiologyNatural sciencesVirology

Identifiers

PMID40612902
PMCPMC12225894

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.