ArticleBiophotonics discovery2025
Label-free method to monitor metabolism during long-term culture of human pluripotent stem cell derived cardiomyocytes.
Article in Biophotonics discovery, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Significance: Human pluripotent stem-cell-derived cardiomyocytes (hPSC-CMs) are a powerful tool for drug discovery, and metabolic changes are associated with their long-term culture and maturation. However, the lack of technologies to monitor hPSC-derived cardiomyocyte metabolism during long-term culture presents a major technical bottleneck. Aim: Efforts to monitor Approach: Label-free optical metabolic imaging (OMI) of autofluorescent metabolic coenzymes was performed at multiple time points during the extended culture maturation process. In addition, OMI monitored hPSC-CMs grown on substrates with varying stiffness and on cardiomyocytes derived from induced pluripotent stem cells associated with cardiac arrhythmia. OMI was paired with immunofluorescence to validate structural maturation. Results: Single-cell OMI can identify metabolic changes during cardiomyocyte maturation through extended Conclusions: Our results demonstrate that label-free OMI can be used to monitor metabolic changes in hPSC-CMs under varying culture conditions in a rapid, non-destructive manner with single-cell resolution, providing insight into metabolic transitions arising from time in culture, culture conditions, or disease states.
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