ArticleFrontiers in veterinary science2025
An SNP-based diagnostic method for
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.
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Who cites it
5 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Epidemiology of Brucellosis Among Deer in China From 1978 to 2025: A Systematic Review and Meta-Analysis.Transboundary and emerging diseases · 2026Pooled it
- Strain-Specific Loci in Bacterial Genomes: Whole-Genome Discovery, Genomic Context, and Application for Multi-Strain qPCR Monitoring.Microorganisms · 2026Article
- Ultrasensitive Detection and Its Potential Applications in the Diagnosis of Brucellosis.Current microbiology · 2026Review
- Development and Evaluation of Multiple Droplet Digital PCR Method for Specific Detection and Differentiation ofAnimals : an open access journal from MDPI · 2026Article
- Prevalence and detection ofFrontiers in cellular and infection microbiology · 2026Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Brucellosis, a zoonotic bacterial infection caused by Objective: To develop a diagnostic method capable of specifically detecting S2 vaccine strain infections. Methods: Two probes were designed targeting single nucleotide polymorphism (SNP) loci upstream of the Results: Both types of methods demonstrated high specificity. However, MGB-SNPdd showed greater sensitivity than other detection methods. Reduction of overlap between the probe sequence and the transient stem-loop structure enhanced detection sensitivity. In the clinical applicability analysis, ddPCR methods exhibited higher rates of S2 vaccine strain detection compared with qPCR methods. Conclusion: SNP-based ddPCR methods demonstrate higher sensitivity than qPCR methods and enable specific detection of brucellosis caused by the S2 vaccine strain. Reduction of probe overlap with the transient stem-loop structure improves detection sensitivity, providing valuable insights for enhanced PCR amplification efficiency.
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Registered trials
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