Evidence map›Paper›PMID 40612073›Full record

ArticleSynthetic biology (Oxford, England)2025

Minimal shuttle vectors for

Lorenzo Scutteri, Patrick Barth, Sahand Jamal Rahi

Abstract read
In one paragraph

Article in Synthetic biology (Oxford, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Lorenzo ScutteriLaboratory of the Physics of Biological Systems, École polytechnique fédérale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland.
Patrick BarthInterfaculty Institute of Bioengineering, École polytechnique fédérale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland.
Sahand Jamal RahiLaboratory of the Physics of Biological Systems, École polytechnique fédérale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland.ORCID https://orcid.org/0000-0001-6442-7087

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sophisticated genetic engineering tasks such as protein domain grafting and multi-gene fusions are hampered by the lack of suitable vector backbones. In particular, many restriction sites are in the backbone outside the polylinker region (multiple cloning site; MCS) and thus unavailable for use, and the overall length of a plasmid correlates with poorer ligation efficiency. To address this need, we describe the design and validation of a collection of six minimal integrating or centromeric shuttle vectors for

Indexed as

de novo synthesisdomain graftingdomain insertionminimal shuttle vectormulti-gene fusionrestriction sitesSaccharomyces cerevisiae

Identifiers

PMID40612073
PMCPMC12224612

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.