Evidence map›Paper›PMID 40610854›Full record

ArticleBMC plant biology2025

Identification of genes involved in verazine biosynthesis in Veratrum grandiflorum and their heterologous production in Saccharomyces cerevisiae.

Kaiyun Hong, Xiangyu Liu, Changqing Wang, Yanjiao Xu, Guisheng Xiang, Shengchao Yang, Jianli Yang, Guanghui Zhang, Simei He

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Reconstruction ofEngineering in life sciences · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kaiyun Hong *College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Xiangyu Liu *College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Changqing WangCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Yanjiao XuCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Guisheng XiangCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Shengchao YangCollege of Biological and Agricultural Sciences, Honghe University, Mengzi, 661199, China.
Jianli YangKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Guanghui ZhangCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China. zgh73107310@163.com.
Simei HeCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China. simeiheynau@163.com.

Funding

National Natural Science Foundation of China 82160727Xingdian Youth talent Project XDYC-QNRC-2022-0209Yunnan Characteristic Plant Extraction Laboratory 2022YKZY001
6 · The paper itself

Abstract

Verazine is a verazine-type alkaloid primarily found in plants of the family Melanthiaceae. In addition to its direct antifungal activity, it serves as a key precursor in the biosynthesis of cyclopamine, a potent inhibitor of the Hedgehog signaling pathway. In this study, we sequenced the transcriptomes of Veratrum grandiflorum roots treated with methyl jasmonate, genes involved in verazine biosynthesis VgCYP90B27, VgCYP94N1, VgCYP90G1, and VgGABAT, were characterized. We produced verazine using Saccharomyces cerevisiae chassis with a yielding 71.62 ± 3.50 µg/L. Furthermore, the GAME4 gene with aldeylation of C-26 was also introduced, and the flask fermentation titer was increased to 175 ± 1.38 µg/L, which was the highest titer reported so far. These results provide a reference for high efficient heterologous biosynthesis study of verazine, and lay a foundation for the deciphering of cyclopamine biosynthetic pathway.

Indexed as

Genes, PlantPlant ProteinsSaccharomyces cerevisiaeVeratrumVeratrum AlkaloidsAcetatesBiosynthetic PathwaysCyclopentanesOxylipinsPlant RootsAcetatesCyclopentanesmethyl jasmonateOxylipinsPlant ProteinsVeratrum AlkaloidsCyclopamineHeterologous biosynthesisSaccharomyces cerevisiaeVeratrum grandiflorumVerazine

Identifiers

PMID40610854
PMCPMC12224848

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.