Evidence map›Paper›PMID 40610593›Full record

ArticleScientific reports2025

Restoration of Oxacillin susceptibility in MRSA strains by NPPB.

Se Jin Jo, Rui Jiang, Sook In Jung, Joon Haeng Rhee, Young Ran Kim

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Se Jin JoCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Chonnam National University, 77 Youngbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea.
Rui JiangCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Chonnam National University, 77 Youngbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea.
Sook In JungChonnam National University Medical School, Gwangju, 61469, Republic of Korea.
Joon Haeng RheeClinical Vaccine R&D Center, Department of Microbiology, Combinatorial Tumor Immunotherapy MRC, Chonnam National University Medical School, Hwasun-gun, Jeonnam, 58128, Republic of Korea.
Young Ran KimCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Chonnam National University, 77 Youngbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea. kimyr@jnu.ac.kr.

Funding

National Research Foundation of Korea (NRF) grant funded by the Korean government 2023R1A2C1005711
6 · The paper itself

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a critical public health issue because of its resistance to multiple antibiotics, including β-lactams such as methicillin and oxacillin. To search for antibacterial sensitizers, we have investigated the synergistic antibacterial effects of the combination of the β-lactam antibiotic oxacillin and 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) against the MRSA strains. Checkerboard assays showed the synergistic antibacterial efficacy of NPPB combined with oxacillin against five MRSA strains. In addition, the combined effect of NPPB and oxacillin was evaluated by disc diffusion assay and time-dependent growth assay in MRSA strain ATCC 33591, and real-time PCR results showed that the combined treatment decreased the expression levels of abcA and norA genes encoding efflux transporters. NPPB also decreased the oxacillin-induced biofilm formation of ATCC 33591. Field emission transmission electron microscopy showed that the combination treatment induced extensive damage to the bacterial cell wall and membrane, leading to cell lysis. NPPB and oxacillin treated to mice for one week did not cause toxicity on the liver and kidney functions. These findings highlighted the potential of combination therapy of oxacillin and NPPB to treat MRSA infection by enhancing antibiotic efficacy and overcoming resistance.

Indexed as

Anti-Bacterial AgentsMethicillin-Resistant Staphylococcus aureusOxacillinAnimalsBacterial ProteinsBiofilmsDrug SynergismMiceMicrobial Sensitivity TestsStaphylococcal InfectionsAnti-Bacterial AgentsBacterial ProteinsOxacillin5-nitro-2-(3-phenylpropylamino) benzoic acidAnti-bacterial sensitizerAntibiotic resistanceMethicillin-resistant Staphylococcus aureusOxacillinSynergistic antibacterial effects

Identifiers

PMID40610593
PMCPMC12229562

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.