Evidence map›Paper›PMID 40610480›Full record

ArticleNPJ biofilms and microbiomes2025

Bacillus secretes nucleases to degrade dsRNA, thereby reducing host's susceptibility to RNAi.

Xinyi Han, Haichao Li, Saibo Xu, Xuexia Miao, Ruobing Guan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NPJ biofilms and microbiomes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Xinyi Han *College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
Haichao Li *State Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
Saibo XuCollege of Plant Protection, Henan Agricultural University, Zhengzhou, China.
Xuexia MiaoState Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
Ruobing GuanCollege of Plant Protection, Henan Agricultural University, Zhengzhou, China. guanruobing@126.com.

Funding

Key scientific research projects of colleges and universities in Henan Province 25B210009Shanghai Agricultural Science and Technology Innovation Program K2023019Shanghai Rising-Star Program 22QB1405900State Key Laboratory of Cotton Bio-breeding and Integrated Utilization and Sponsored by State Key Laboratory of Cotton Bio-breeding and Integrated Utilization Open Fund CB2025A34the Science and Technology Research Project of Henan Province 252102111110
6 · The paper itself

Abstract

RNAi technology, which can induce mortality by disrupting the transcription of essential growth and development-related genes in insects, has emerged as a groundbreaking pest control method. However, insects have developed defense mechanisms to counteract the efficiency of RNAi. The specific role of symbiotic microorganisms in this process remains poorly understood and requires further exploration. This study examines the reduced RNAi efficiency in Lepidopteran pest Helicoverpa armigera. Through screening, six Bacillus strains exhibiting dsRNA-degrading activity were identified through in vitro assays. Further investigation into one representative strain Ba 6 revealed that it significantly decreased RNAi efficiency by secreting ribonuclease into the insect gut fluid, directly degrading dsRNA, thus reducing its accumulation and blocking RNAi effects. These findings clarify the mechanism by which symbiotic bacteria influence the host's RNAi efficiency and provides a valuable reference for the development and large-scale implementation of RNA biopesticides targeting H. armigera and other lepidopteran pests.

Indexed as

BacillusMothsRibonucleasesRNA, Double-StrandedRNA InterferenceAnimalsSymbiosisRibonucleasesRNA, Double-Stranded

Identifiers

PMID40610480
PMCPMC12229549

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.