Evidence map›Paper›PMID 40608275›Full record

ArticleMolecular biotechnology2026

Extracellular Expression and Diagnostic Potential of Dengue Virus Type 3 E Protein Domain III Through Codon Optimization in Komagataella phaffii.

Camila Marques de Simone, Maria Carolina Yi Lin Lee, Yasmin Mayra Bispo, Marcos Benedito Adão, Leonardo de Paiva Turetta, Marita Vedovelli Cardozo, Monalessa Fábia Pereira, Christiane Eliza Motta Duarte, Sérgio Oliveira De Paula, Caio Roberto Soares Bragança

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Camila Marques de SimoneLaboratory of Microorganism Physiology, Department of Biomedical Sciences and Health, Universidade do Estado de Minas Gerais (UEMG), Rua Sabará, 164, Centro, Passos, MG, CEP: 37900-004, Brazil.
Maria Carolina Yi Lin LeeLaboratory of Microorganism Physiology, Department of Biomedical Sciences and Health, Universidade do Estado de Minas Gerais (UEMG), Rua Sabará, 164, Centro, Passos, MG, CEP: 37900-004, Brazil.
Yasmin Mayra BispoLaboratory of Microorganism Physiology, Department of Biomedical Sciences and Health, Universidade do Estado de Minas Gerais (UEMG), Rua Sabará, 164, Centro, Passos, MG, CEP: 37900-004, Brazil.
Marcos Benedito AdãoLaboratory of Microorganism Physiology, Department of Biomedical Sciences and Health, Universidade do Estado de Minas Gerais (UEMG), Rua Sabará, 164, Centro, Passos, MG, CEP: 37900-004, Brazil.
Leonardo de Paiva TurettaLaboratory of Microorganism Physiology, Department of Biomedical Sciences and Health, Universidade do Estado de Minas Gerais (UEMG), Rua Sabará, 164, Centro, Passos, MG, CEP: 37900-004, Brazil.
Marita Vedovelli CardozoLaboratory of Microorganism Physiology, Department of Biomedical Sciences and Health, Universidade do Estado de Minas Gerais (UEMG), Rua Sabará, 164, Centro, Passos, MG, CEP: 37900-004, Brazil.
Monalessa Fábia PereiraLaboratory of Microorganism Physiology, Department of Biomedical Sciences and Health, Universidade do Estado de Minas Gerais (UEMG), Rua Sabará, 164, Centro, Passos, MG, CEP: 37900-004, Brazil.
Christiane Eliza Motta DuarteLaboratory of Cell Signaling and Glycobiology, Department of Biomedical Sciences and Health, Universidade do Estado de Minas Gerais (UEMG), Rua Sabará, 164, Centro, Passos, MG, CEP: 37900-004, Brazil.
Sérgio Oliveira De PaulaLaboratory of Molecular Virology and Immunology, Department of General Biology, Universidade Federal de Vicosa (UFV), Av. Peter Henry Rolfs s/n, Viçosa, MG, Brazil.
Caio Roberto Soares BragançaLaboratory of Microorganism Physiology, Department of Biomedical Sciences and Health, Universidade do Estado de Minas Gerais (UEMG), Rua Sabará, 164, Centro, Passos, MG, CEP: 37900-004, Brazil. caio.braganca@uemg.br.ORCID http://orcid.org/0009-0000-7360-6562

Funding

Universidade do Estado de Minas Gerais Bolsa de Produtividade/Projeto de Pesquisa Estruturante
6 · The paper itself

Abstract

Dengue is a mosquito-borne viral disease caused by the dengue virus (DENV), which affects millions worldwide. The envelope (E) protein, particularly domain III (EDIII), plays a critical role in viral entry and is a major target for diagnostic and vaccine development. In this study, we aimed to express and purify EDIII from DENV-3 using Komagataella phaffii as an expression system. The recombinant gene was codonoptimized, cloned into the pPICZαA vector, and integrated into the K. phaffii genome. Protein expression was induced with methanol and purified using Ni

Indexed as

DengueDengue VirusViral Envelope ProteinsAnimalsAntibodies, ViralCloning, MolecularCodonEnzyme-Linked Immunosorbent AssayHumansImmunoglobulin GImmunoglobulin MProtein DomainsRecombinant ProteinsAntibodies, ViralCodonImmunoglobulin GImmunoglobulin MRecombinant ProteinsViral Envelope ProteinsBioprocessCodon optimizationDengueDENVDomain III (EDIII)Heterologous expressionRecombinant proteinYeast

Identifiers

PMID40608275

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.