Genome-wide CRISPR knockout screen identifies activating transcription factor (ATF1) as an activator of HIV gene expression.

Alona Kuzmina, Seraj Wattad, Praveenkumar Murugavelu, Noa Amir, Nili Tickotsky, Liron Levin, Ran Taube

Abstract read

In one paragraph

Article in mBio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Alona Kuzmina *The Shraga Segal Department of Microbiology Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Be'er Sheva, Israel.

Seraj Wattad *The Shraga Segal Department of Microbiology Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Be'er Sheva, Israel.

Praveenkumar Murugavelu *The Shraga Segal Department of Microbiology Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Be'er Sheva, Israel.

Noa AmirThe Shraga Segal Department of Microbiology Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Be'er Sheva, Israel.

Nili TickotskyBioinformatics Core Facility, Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Be'er Sheva, Israel.

Liron LevinBioinformatics Core Facility, Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Be'er Sheva, Israel.

Ran TaubeThe Shraga Segal Department of Microbiology Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Be'er Sheva, Israel.ORCID 0000-0002-2062-4537

Funding

All of Us Research Program (All of Us NIH) 5R21AI270197Deutsche Forschungsgemeinschaft 508136175Israel Science Foundation 1884/24United States-Israel Binational Science Foundation 2021273

6 · The paper itself

Abstract

Antiretroviral therapy against the human immunodeficiency virus (HIV) has significantly prolonged the life span of people living with HIV, transforming viral infection into a latent condition that is characterized with undetectable viral loads. Yet, a complete cure of infection is out of reach, as transcriptionally silent but replication-competent proviruses persist in a long-lived reservoir that is resistant to therapy. The current work follows a genome-wide CRISPR knockout screen in human CD4 IMPORTANCE: HIV persists in resting CD4

Indexed as

Activating Transcription Factor 1Gene Expression Regulation, ViralHIV-1CD4-Positive T-LymphocytesClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsGene Knockout TechniquesHIV InfectionsHumansPromoter Regions, GeneticReceptors, CCR5Virus LatencyActivating Transcription Factor 1ATF1 protein, humanCCR5 protein, humanReceptors, CCR5activating factor (ATF1)CCR5 antisense lncRNACCR5 co-receptorHIV gene transcription regulationlatency

Identifiers

PMID40607797

DOI10.1128/mbio.00557-25

PMCPMC12345191

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.