Evidence map›Paper›PMID 40607341›Full record

ArticleFrontiers in veterinary science2025

Investigation of truncated replication protein mutant of Canine Circovirus: synergistic interaction with Feline Panleukopenia Virus.

Suyao Li, Jialiang Xin, Yi Peng, Haifeng Liu, Ziyao Zhou, Zhijun Zhong, Hualin Fu, Min Yang, Wancheng Li, Ruihu Wu and 1 more

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Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Suyao Li *The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Jialiang Xin *The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Yi PengCollege of Landscape Architecture, Sichuan Agricultural University, Chengdu, China.
Haifeng LiuThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Ziyao ZhouThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Zhijun ZhongThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Hualin FuInnovative Engineering Research Center of Veterinary Pharmaceutics, Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Min YangPet Nutrition and Health Research Center, Chengdu Agricultural College, Chengdu, China.
Wancheng LiThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Ruihu WuThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Guangneng PengThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Canine Circovirus (CanineCV) is a non-enveloped, single-stranded circular DNA virus in the Method: We constructed and validated clones and plasmids for CanineCV/ SC49 (which carries the normal Rep gene) and CanineCV/SC50 (which carries the truncated Rep'gene). Virus particles were visualized using transmission electron microscopy (TEM), while quantitative polymerase chain reaction (qPCR) assessed viral load. Additionally, we examined the effects of Rep and Rep' proteins on cellular viability, their roles in FPV replication, and the host interferon type I (IFN-I) response. Results: The Rep' protein significantly enhances the cytotoxicity of CanineCV against the F81 cell line, outperforming the Rep protein in this regard. However, when assessing the proliferation-promoting effects on FPV, both proteins demonstrated positive effects, but Rep exhibited a significantly greater impact than Rep' Additionally, qPCR analysis revealed that Rep has a stronger inhibitory effect on the expression of IFN-α, IFN-β, MxA, and ISG15 genes compared to Rep'. Conclusion: This study underscores the dual roles of Canine Circovirus in modulating host cell viability. On one hand, it enhances the replication of co-infecting viruses; on the other hand, it suppresses the host's antiviral responses. These findings provide valuable insights into the pathogenic mechanisms of Canine Circovirus.

Indexed as

Canine CircovirusFeline Panleukopenia Virusreplication proteinsynergistic effecttruncated replication protein

Identifiers

PMID40607341
PMCPMC12213266

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.