ArticleFrontiers in nutrition2025
Low concentration press cake protein isolates preserve biological activity during lyophilization and spray drying.
Article in Frontiers in nutrition, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Highly Sustainable Plant and Yeast Hydrolysate-Based Alternative for Basal Medium Supports Long-TermFoods (Basel, Switzerland) · 2026Article
- Towards Cost-Effective and Sustainable Media Formulations for Terrestrial and Aquatic Cellular Agriculture.Foods (Basel, Switzerland) · 2026Review
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Authors and funding
6 authors.
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Abstract
A primary challenge in bringing cultivated meat to the market is the high cost of the cell culture media, largely due to their reliance on serum albumins. The production of these albumins is anticipated to become a major bottleneck of this industry. Recently, human serum albumin (HSA) was successfully substituted with seed protein isolates from press cakes enriched with plant albumins. However, these isolates require storage at -80°C to maintain activity, as long-term storage at 4°C or lyophilization leads to aggregation and loss of biological activity. Here, we show that concentrated protein isolates from Styrian oil pumpkin can effectively substitute for human serum albumin (HSA) and support higher proliferation rates in short term experiments as compared to HSA when stored at -80°C (
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