Evidence map›Paper›PMID 40604462›Full record

ArticleBMC plant biology2025

The mechanism of PGR-free direct organogenesis in Lycium ruthenicum leaf explants revealed by RNA-Seq and phytohormone metabolome co-analysis.

Lujia Li, Yucheng Wang, Wenhui Liu, Yue Gao, Jiawen Wang, Huifang Lu, Weiman Xu, Qin-Mei Wang

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Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Lujia LiKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Yucheng WangKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Wenhui LiuKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Yue GaoKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Jiawen WangKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Huifang LuKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Weiman XuKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China.
Qin-Mei WangKey Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, College of Forestry, Shenyang Agricultural University, Shenyang, 110866, China. wqmwan@syau.edu.cn.

Funding

National Natural Science Foundation of China 32171831Scientific Research Fund of Liaoning Provincial Education Department JYTYB2024040
6 · The paper itself

Abstract

backgroundLycium ruthenicum is an economically important shrub known for its resistance to drought and saline-alkali conditions. This study identified a particular L. ruthenicum clone whose leaf explants can undergo direct organogenesis on plant growth regulator (PGR)-free media, and both rooting and shooting abilities of the leaf-tip explants were significantly greater than those of the leaf-middle explants. However, the underlying mechanisms remain unclear.

resultsRNA-Seq analysis revealed that the differentially expressed genes (DEGs) associated with rooting and shooting in both leaf-tip and leaf-middle explants were enriched in the Plant hormone signal transduction KEGG pathway. For the first time, we identified 16 and nine differentially accumulated metabolites (DAMs) linked to direct root and shoot organogenesis from leaf explants, respectively. The stronger direct rooting ability observed in leaf-tip explants was associated with (i) up-regulation of sucrose synthase (SUS), scarecrow-like 21 (SCL21), transport inhibitor response 1 (TIR1), and auxin response factor (ARF) genes; (ii) up-regulation of IAA, IAA-Leu-Me, and BAP; (iii) down-regulation of eight DAMs. Moreover, the enhanced direct shooting ability in leaf-tip explants was correlated with (i) 13 DAMs including upregulated JA and JA-ILE, (ii) up-regulated SUS and (iii) down-regulated jasmonate ZIM domain 2 (JAZ2) gene. Notably, JAZ2 and these 13 DAMs represent newly discovered factors associated with the stronger shooting ability of leaf explants. Additionally, this study conducted correlation analyses between DAMs and DEGs related to rooting and shooting in leaf explants, as well as the enhanced rooting and shooting capacities of leaf-tip explants.

conclusionsThis study identifies the key DEGs and DAMs correlated with the direct organogenesis of L. ruthenicum leaf explants. Building upon these findings, a mechanistic model for the stronger direct organogenesis observed in leaf-tip explants was established. These findings offer valuable theoretical guidance for optimizing direct organogenesis systems in plant leaves.

Indexed as

LyciumMetabolomeOrganogenesis, PlantPlant Growth RegulatorsPlant LeavesGene Expression Regulation, PlantPlant RootsRNA-SeqPlant Growth RegulatorsBlack wolfberryDirect organogenesisLeaf explantPlant hormoneTranscriptome

Identifiers

PMID40604462
PMCPMC12220515

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.