Evidence map›Paper›PMID 40604453›Full record

ArticleBMC plant biology2025

Physiological and transcriptomic analyses reveal the regulatory mechanisms for the adaptation of Quercus robur to shade conditions.

Xiong Huang, Qiuhong Hu, Mengke Dou, Cai Liu, Jiangtao Fan, Jia Liu, Peng Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xiong Huang *National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.
Qiuhong Hu *National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.
Mengke Dou *National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.
Cai LiuSichuan Forestry and Grassland Science and Technology Extension Station, Chengdu, 610081, China.
Jiangtao FanNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.
Jia LiuNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.
Peng ZhuNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China. zhup@sicau.edu.cn.

Funding

Sichuan Provincial Science and Technology Support Program 2024YFNH0028
6 · The paper itself

Abstract

backgroundAs an important multipurpose tree species, the growth and development of Quercus robur are significantly influenced by shading conditions. Although the critical role of shading in plant growth and development has been well established, the responses of oak trees to shading remain poorly understood. This study aimed to investigate the physiological and transcriptomic changes in Q. robur under varying shade conditions.

resultsShade had a significant impact on the growth and physiological indicators of Q. robur. As the shading level increased, the plant height, ground diameter, Pn, PRO content, and SOD activity of Q. robur initially increased before subsequently decreasing. In contrast, CHL-a, CHL-b, Car content, SP content, and POD activity exhibited an increasing trend. Transcriptome sequencing was performed on Q. robur samples subjected to different shade treatments: no shade (T1), 40% shade (T3), and 80% shade (T5). Following rigorous quality control, we obtained 57.62 Gb of high-quality sequencing data, and 3,795 DEGs (T1-VS-T3 group), 7,852 DEGs (T1-VS-T5 group), and 7,139 DEGs (T3-VS-T5 group) were identified. KEGG pathway analyses of 978 overlapping DEGs identified crucial involvement of photosynthesis-antenna proteins and plant hormone signal transduction pathways. RT-qPCR validation of six DEGs confirmed the reliability of the transcriptome sequencing data. Notably, we demonstrated significant associations (p < 0.05) between 516 core genes and key growth-physiological traits by correlation analysis.

conclusionsThis study provides insights into the patterns of growth changes and physiological responses in Q. robur under shading conditions. Q. robur has adapted to shade conditions by inhibiting photosynthesis, synthesizing chlorophyll and Car, activating the antioxidant system, and regulating osmoregulatory substances. Furthermore, this study elucidated the transcriptional responses of Q. robur to shading, thereby revealing the molecular mechanisms underlying shade adaptation. This research provides a foundational understanding of physiological and molecular mechanisms of Q. robur in shade conditions.

Indexed as

Adaptation, PhysiologicalQuercusSunlightTranscriptomeGene Expression ProfilingGene Expression Regulation, PlantLightPhotosynthesisPhotosynthesisPlant hormoneQuercus roburShade conditionsTranscriptome analysis

Identifiers

PMID40604453
PMCPMC12220647

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.